CHST3 recombinant protein

CHST3 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122836

• Gene Names: Chst3; C6ST; GST-0; C6ST-1
• Purity: >95% as determined by SDS-PAGE
• Synonyms: CHST3; CHST3 Protein; Mouse; Recombinant (His Tag); Mouse CHST3/C6ST-1 Protein (His Tag); C6ST Protein; C6ST-1 Protein; GST-0 Protein; carbohydrate (chondroitin 6) sulfotransferase 3; CHST3 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Glu39-Thr472

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse CHST3 (NP_058083.2)(Glu 39-Thr 472) was fused with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CHST3 recombinant protein

Background: Carbohydrate sulfotransferase 3, also known as Chondroitin 6-O-sulfotransferase 1, Chondroitin 6-sulfotransferase and CHST3, is a single-pass type II membrane protein which belongs to thesulfotransferase 1 family and Gal/GlcNAc/GalNAc subfamily. CHST3 is widely expressed in adult tissues. It is expressed in heart, placenta, skeletal muscle and pancreas. CHST3 is also expressed in various immune tissues such as spleen, lymph node, thymus and appendix. CHST3 catalyzes the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. It is a chondroitin sulfate which constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. It can also sulfate Gal residues of keratan sulfate, another glycosaminoglycan, and the Gal residues in sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides. It may play a role in the maintenance of naive T-lymphocytes in the spleen. Defects in CHST3 are the cause of spondyloepiphyseal dysplasia Omani type (SED Omani type) which is an autosomal recessive disorder characterized by normal length at birth but severely reduced adult height (11-13 cm), severe progressive kyphoscoliosis, arthritic changes with joint dislocations, genu valgum, cubitus valgus, mild brachydactyly, camptodactyly, microdontia and normal intelligence. As a consequence of the arthropathy and the contractures, affected individuals develop restricted joint movement. Defects in CHST3 are also a cause of humerospinal dysostosis (HSD) which is characterized by bifurcation of the ends of the humerus, subluxation in the elbow joints, widened iliac bones, talipes equinovarus and coronal cleft vertebrae. Congenital, progressive heart disease, possibly with fatal outcome, is observed in some patients.

References

Fukuta M., et al., 1998, Biochim. Biophys. Acta 1399:57-61. Tsutsumi K., et al., 1998, FEBS Lett. 441:235-241. Thiele H., et al., 2004, Proc. Natl. Acad. Sci. USA. 101:10155-10160. Hermanns P., et al., 2008, Am. J. Hum. Genet. 82:1368-1374.

NCBI and Uniprot Product Information

• NCBI GI #: 256985176
• NCBI GeneID: 53374
• NCBI Accession #: NP_058083.2
• NCBI GenBank Nucleotide #: NM_016803.3
• UniProt Accession #: O88199; Q794I5
• Molecular Weight: 53,997 Da
• NCBI Official Full Name: carbohydrate sulfotransferase 3
• NCBI Official Synonym Full Names: carbohydrate (chondroitin 6/keratan) sulfotransferase 3
• NCBI Official Symbol: Chst3
• NCBI Official Synonym Symbols: C6ST; GST-0; C6ST-1
• NCBI Protein Information: carbohydrate sulfotransferase 3
• UniProt Protein Name: Carbohydrate sulfotransferase 3
• Protein Family: Carbohydrate sulfotransferase
• UniProt Gene Name: Chst3
• UniProt Synonym Gene Names: C6st; Gst0; C6ST-1; GST-0

Uniprot Description

CHST3: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Can also sulfate Gal residues of keratan sulfate, another glycosaminoglycan, and the Gal residues in sialyl N- acetyllactosamine (sialyl LacNAc) oligosaccharides. May play a role in the maintenance of naive T-lymphocytes in the spleen. Defects in CHST3 are a cause of spondyloepiphyseal dysplasia with congenital joint dislocations (SEDC-JD). A bone dysplasia clinically characterized by dislocation of the knees and/or hips at birth, clubfoot, elbow joint dysplasia with subluxation and limited extension, short stature, and progressive kyphosis developing in late childhood. The disorder is usually evident at birth, with short stature and multiple joint dislocations or subluxations that dominate the neonatal clinical and radiographic picture. During childhood, the dislocations improve, both spontaneously and with surgical treatment, and features of spondyloepiphyseal dysplasia become apparent, leading to arthritis of the hips and spine with intervertebral disk degeneration, rigid kyphoscoliosis, and trunk shortening by late childhood. Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.

Protein type: EC 2.8.2.17; Glycan Metabolism - chondroitin sulfate biosynthesis; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: 10|10 B4

Cellular Component: Golgi apparatus; Golgi membrane; integral component of membrane; membrane

Molecular Function: chondroitin 6-sulfotransferase activity; N-acetylglucosamine 6-O-sulfotransferase activity; proteoglycan sulfotransferase activity; sulfotransferase activity; transferase activity

Biological Process: carbohydrate metabolic process; chondroitin sulfate biosynthetic process; positive regulation of cellular component movement; sulfur compound metabolic process; T cell homeostasis