Rat Acetylcholine receptor subunit delta ELISA Kit | CHRND elisa kit

Rat Acetylcholine receptor subunit delta (CHRND) ELISA Kit

Cat. Num. AAA280860

• Reactivity: Rat
• Synonyms: Acetylcholine receptor subunit delta; Rat Acetylcholine receptor subunit delta (CHRND) ELISA Kit; CHRND elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat CHRND. No significant cross-reactivity or interference between Rat CHRND and analogues was observed.

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for CHRND elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate CHRND in samples. An antibody specific for CHRND has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCHRND present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHRND is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHRND bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 9506487
• NCBI GeneID: 54240
• NCBI Accession #: NP_062171.1
• NCBI GenBank Nucleotide #: NM_019298.1
• UniProt Accession #: P25110
• Molecular Weight: 58,884 Da
• NCBI Official Full Name: acetylcholine receptor subunit delta
• NCBI Official Synonym Full Names: cholinergic receptor nicotinic delta subunit
• NCBI Official Symbol: Chrnd
• NCBI Protein Information: acetylcholine receptor subunit delta
• UniProt Protein Name: Acetylcholine receptor subunit delta
• Protein Family: Acetylcholine receptor
• UniProt Gene Name: Chrnd
• UniProt Synonym Gene Names: Acrd

NCBI Description

delta subunit of the nicotinic acetylcholine receptor; can assemble into functionally different acetylcholine receptor channel subtypes [RGD, Feb 2006]

Uniprot Description

nAChRD: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Defects in CHRND are a cause of multiple pterygium syndrome lethal type (MUPSL). Multiple pterygia are found infrequently in children with arthrogryposis and in fetuses with fetal akinesia syndrome. In lethal multiple pterygium syndrome there is intrauterine growth retardation, multiple pterygia, and flexion contractures causing severe arthrogryposis and fetal akinesia. Subcutaneous edema can be severe, causing fetal hydrops with cystic hygroma and lung hypoplasia. Oligohydramnios and facial anomalies are frequent. Defects in CHRND are a cause of congenital myasthenic syndrome slow-channel type (SCCMS). SCCMS is the most common congenital myasthenic syndrome. Congenital myasthenic syndromes are characterized by muscle weakness affecting the axial and limb muscles (with hypotonia in early-onset forms), the ocular muscles (leading to ptosis and ophthalmoplegia), and the facial and bulbar musculature (affecting sucking and swallowing, and leading to dysphonia). The symptoms fluctuate and worsen with physical effort. SCCMS is caused by kinetic abnormalities of the AChR, resulting in prolonged endplate currents and prolonged AChR channel opening episodes. Defects in CHRND are a cause of congenital myasthenic syndrome fast-channel type (FCCMS). FCCMS is a congenital myasthenic syndrome characterized by kinetic abnormalities of the AChR. In most cases, FCCMS is due to mutations that decrease activity of the AChR by slowing the rate of opening of the receptor channel, speeding the rate of closure of the channel, or decreasing the number of openings of the channel during ACh occupancy. The result is failure to achieve threshold depolarization of the endplate and consequent failure to fire an action potential. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Delta/CHRND sub-subfamily.

Protein type: Channel, ligand-gated; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 9q35

Cellular Component: acetylcholine-gated channel complex; cell junction; plasma membrane; postsynaptic membrane

Molecular Function: acetylcholine binding; acetylcholine-gated cation channel activity; ligand-gated ion channel activity

Biological Process: cation transport; excitatory postsynaptic potential; musculoskeletal movement; neuromuscular synaptic transmission; regulation of membrane potential; response to nicotine; skeletal muscle contraction; skeletal muscle tissue growth; synaptic transmission, cholinergic