Cholinergic Receptor, Nicotinic, Beta 3 Recombinant Protein | CHRNb3 recombinant protein

Recombinant Cholinergic Receptor, Nicotinic, Beta 3 (CHRNb3)

Cat. Num. AAA2033460

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Cholinergic Receptor; Nicotinic; Beta 3; Recombinant Cholinergic Receptor; Beta 3 (CHRNb3); CHR-NB3; CHRN-B3; N-AChRB3; NAChRB3; N-AChR-B3; Neuronal Acetylcholine Receptor Beta 3; CHRNb3 recombinant protein

• Host: E Coli
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: EN ADGRFEGSLM TKVIVKSNGT VVWTPPASYKSSCTMDVTFF PFDRQNCSMK FGSWTYDGTM VDLILINENV DRKDFFDNGEWEILNAKGMK GNRRDGVYSY PFITYSFVLR
• Sequence Length: 458

• Applicable Applications for CHRNb3 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 16.6kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Glu119~Arg230
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 119583612
• NCBI GeneID: 1142
• Molecular Weight: 52,729 Da
• NCBI Official Full Name: cholinergic receptor, nicotinic, beta 3
• NCBI Official Synonym Full Names: cholinergic receptor nicotinic beta 3 subunit
• NCBI Official Symbol: CHRNB3
• NCBI Protein Information: neuronal acetylcholine receptor subunit beta-3
• UniProt Protein Name: Neuronal acetylcholine receptor subunit beta-3
• Protein Family: Neuronal acetylcholine receptor
• UniProt Gene Name: CHRNB3
• UniProt Entry Name: ACHB3_HUMAN

NCBI Description

The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are (hetero)pentamers composed of homologous subunits. The subunits that make up the muscle and neuronal forms of nAChRs are encoded by separate genes and have different primary structure. There are several subtypes of neuronal nAChRs that vary based on which homologous subunits are arranged around the central channel. They are classified as alpha-subunits if, like muscle alpha-1 (MIM 100690), they have a pair of adjacent cysteines as part of the presumed acetylcholine binding site. Subunits lacking these cysteine residues are classified as beta-subunits (Groot Kormelink and Luyten, 1997 [PubMed 9009220]). Elliott et al. (1996) [PubMed 8906617] stated that the proposed structure for each subunit is a conserved N-terminal extracellular domain followed by 3 conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region.[supplied by OMIM, Apr 2010]

Uniprot Description

nAChRB3: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta- 3/CHRNB3 sub-subfamily.

Protein type: Channel, ligand-gated; Channel, cation; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 8p11.2

Cellular Component: cell junction; integral to membrane; neuron projection; nicotinic acetylcholine-gated receptor-channel complex; plasma membrane; postsynaptic membrane

Molecular Function: acetylcholine binding; acetylcholine receptor activity; channel activity; drug binding; nicotinic acetylcholine-activated cation-selective channel activity

Biological Process: neuromuscular synaptic transmission; protein heterooligomerization; response to nicotine; signal transduction; synaptic transmission; synaptic transmission, cholinergic; transmembrane transport; transport