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Rat Acetylcholine receptor subunit beta ELISA Kit | CHRNB1 elisa kit

Rat Acetylcholine receptor subunit beta (CHRNB1) ELISA Kit

Gene Names
Chrnb1; Acrb; nAChR; RNACRB1
Reactivity
Rat
Synonyms
Acetylcholine receptor subunit beta; Rat Acetylcholine receptor subunit beta (CHRNB1) ELISA Kit; CHRNB1 elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat CHRNB1. No significant cross-reactivity or interference between Rat CHRNB1 and analogues was observed.
Sequence Length
501
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for CHRNB1 elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate CHRNB1 in samples. An antibody specific for CHRNB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCHRNB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHRNB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHRNB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
57,027 Da
NCBI Official Full Name
acetylcholine receptor subunit beta
NCBI Official Synonym Full Names
cholinergic receptor nicotinic beta 1 subunit
NCBI Official Symbol
Chrnb1
NCBI Official Synonym Symbols
Acrb; nAChR; RNACRB1
NCBI Protein Information
acetylcholine receptor subunit beta
UniProt Protein Name
Acetylcholine receptor subunit beta
Protein Family
UniProt Gene Name
Chrnb1
UniProt Synonym Gene Names
Acrb

NCBI Description

beta subunit of the nicotinic acetylcholine receptor; can assemble into functionally different acetylcholine receptor channel subtypes [RGD, Feb 2006]

Uniprot Description

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

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Product Notes

The Rat CHRNB1 chrnb1 (Catalog #AAA283305) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA283305 ELISA Kit recognizes Rat CHRNB1. It is sometimes possible for the material contained within the vial of "Acetylcholine receptor subunit beta, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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