Growth hormone receptor Active Protein | GHR active protein

Growth hormone receptor, 19-264aa, Human, His tag, Baculovirus (Bioactivity Validated)

Cat. Num. AAA206337

• Gene Names: GHR; GHBP; GHIP
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: Growth hormone receptor; 19-264aa; Human; His tag; Baculovirus (Bioactivity Validated); GHR; GHBP; GHIP; GHR active protein

• Host: Baculovirus
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 1mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: FSGSEATAAI LSRAPWSLQS VNPGLKTNSS KEPKFTKCRS PERETFSCHW TDEVHHGTKN LGPIQLFYTR RNTQEWTQEW KECPDYVSAG ENSCYFNSSF TSIWIPYCIK LTSNGGTVDE KCFSVDEIVQ PDPPIALNWT LLNVSLTGIH ADIQVRWEAP RNADIQKGWM VLEYELQYKE VNETKWKMMD PILTTSVPVY SLKVDKEYEV RVRSKQRNSG NYGEFSEVLY VTLPQMSQFT CEEDFYLEHH HHHH
• Sequence Length: 638

• Applicable Applications for GHR active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Biological Activity: Measured by ability to inhibit GH-induced proliferation assay using Nb2-11 Rat lymphoma cells in the presence of 1.25ng/ml of human growth hormone. The ED50 for this effect is equal or less than 10ng/ml.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for GHR active protein

GHR, also known as growth hormone receptor, is a member of the cytokine receptor family. It binds two receptor molecules and thereby induces signal transduction through receptor dimerization. At high concentrations, growth hormone acts as an antagonist because of a large difference in affinities at the respective binding sites. This antagonist action can be enhanced further by reducing binding in the low affinity binding site. Recombinant human GHR, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for GHR active protein

Signal Transduction; Hormones & Cytokines

References

Yang N., et al. (2007) Mol Endocrinol. 21:1642-1655.; Ross RJ., et al. (1997) Mol Endocrinol. 11:265-273.

NCBI and Uniprot Product Information

• NCBI GI #: 4503993
• NCBI GeneID: 2690
• NCBI Accession #: NP_000154
• NCBI GenBank Nucleotide #: NM_000163.4
• UniProt Accession #: P10912; Q9HCX2
• Molecular Weight: 29.4kDa (254aa) 28-40kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: growth hormone receptor isoform 1
• NCBI Official Synonym Full Names: growth hormone receptor
• NCBI Official Symbol: GHR
• NCBI Official Synonym Symbols: GHBP; GHIP
• NCBI Protein Information: growth hormone receptor
• UniProt Protein Name: Growth hormone receptor
• Protein Family: Growth hormone receptor
• UniProt Gene Name: GHR
• UniProt Synonym Gene Names: GH receptor; GH-binding protein; GHBP

NCBI Description

This gene encodes a member of the type I cytokine receptor family, which is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature. In humans and rabbits, but not rodents, growth hormone binding protein (GHBP) is generated by proteolytic cleavage of the extracellular ligand-binding domain from the mature growth hormone receptor protein. Multiple alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jun 2011]

Uniprot Description

Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway ().