Cholinergic Receptor Recombinant Protein | CHRNa7 recombinant protein

Recombinant Cholinergic Receptor, Nicotinic, Alpha 7 (CHRNa7)

Cat. Num. AAA2029810

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation, Immunoprecipitation
• Purity: >90%
• Synonyms: Cholinergic Receptor; Recombinant Cholinergic Receptor; Nicotinic; Alpha 7 (CHRNa7); CHRNa7 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >90%
• Form/Format: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; GEFQRKLY KELVKNYNPL ERPVANDSQP LTVYFSLSLL QIMDVDEKNQ VLTTNIWLQM SWTDHYLQWN VSEYPGVKTV RFPDGQIWKP DILLYNSADE RFDATFHTNV LVNSSGHCQY LPPGIFKSSC YIDVRWFPFD VQHCKLKFGS WSYGGWSLDL QMQEADISGY IPNGEWDLVG IPGKRSERFY ECCKEPYPDV TFTVTMRRRT LYYGLNLLIP CVLISALALL VFLLPADSGE KISLGITVLL SLTVFMLLVA EIMPATSDSV PLIAQYFAST MIIVGLSVVV TVIVLQYHHH DPDGGKMPKW TRVILLNWCA WFLRMKRPGE DKVRPACQHK QRRCSLASVE MSAVAPPPAS NGNLLYIGFR GLDGVHCVPT PDSGVVCGRM ACSPTHDEHL LHGGQPPEGD PDLAKILEEV RYIANRFRCQ DESEAVCSEW KFAACVVDRL CLMAFSVFTI ICTIGILMSAPNFVEAVSKD FA

• Applicable Applications for CHRNa7 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), CoIP, ReporterAssays, Purification, Amine Reactive Labeling.; (May be suitable for use in other assays to be determined by the end user.)
• Predicted isoelectric point: 5.9
• Predicted Molecular Mass: 84.2kDa
• Accurate Molecular Mass: 84kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Residues: Gly23~Ala502
• Tags: Two N-terminal Tags, His-tag and GST-tag
• Tissue Specificity: Salivary Gland, Colon, Kidney, Seminal Vesicle, Ovary.
• Subcellular Location: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane.
• Traits: Freeze-dried powder
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page