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BAFF-CD8a, Fusion Protein

BAFF-CD8a, Fusion Protein

Applications
ELISA, Flow Cytometry, Functional Assay
Purity
Affinity Purified
Purified by affinity chromatography.
Synonyms
BAFF-CD8a; Fusion Protein; Fusion Protein protein
Ordering
For Research Use Only!
Host
CHO cells
Purity/Purification
Affinity Purified
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in 50mM Sodium Phosphate pH 7.5, 100mM Potassium Chloride, 150mM NaCl, 0.5mg/ml Gentamicin Sulfate.
Applicable Applications for BAFF-CD8a, Fusion Protein protein
ELISA (EL/EIA), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Flow Cytometry and ELISA.
Molecular Structure
A soluble molecule consisting of 151 (VQGPEET...c-term) aminoacids of the extracellular domain of human BAFF fused to the extracellular domain (167aa) of murine CD8 alpha, with a predicted monomeric weight of 38.5kD. A portion of the extracellular region of BAFF was truncated to eliminate a potential protease cleavage site.
Functional Application
Human BAFF(trn)-muCD8 binds to human cell surface TACI/BCMA/BAFFR.
Human BAFFtrn (TALL-1, Blys, THANK)-muCD8 Fusion Protein*
Transfectant Cell Line
CHO
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for BAFF-CD8a, Fusion Protein protein
The human B cell activating factor (BAFF) and APRIL(a proliferation inducing ligand) are both type II molecules belonging to the TNF superfamily (TNFSFL #13b and 13 respectively). They are expressed by non-B cells, and are down regulated by mitogenic stimulation(2). BAFF and APRIL bind to at least two receptors: TACI (transmembrane activator and CAML-interactor) and BCMA (B cell maturation antigen), both of which are restricted to B cells (3,4). Ligation of these receptors with recombinant BAFF dramatically increases IgM production by peripheral blood B cells(1). Recently a third receptor for BAFF (BAFF-R) was described(5). BAFF and BAFFR knockout mice have a reduced numbers of mature B cells in the periphery, however TACI and BCMA knockouts do not share this phenotype, suggesting that BAFF-R may the primary receptor for BAFF in mice (8,9,10). Cell surface BAFF can be proteolytically cleaved to form a soluble trimeric molecule (2). Levels of soluble BAFF correspond with levels of autoantibodies in Sjogren's Syndrome(11). Recombinant BAFF(trn)-muCD8 binds to human cell surface TACI/BCMA/BAFFR.
Product Categories/Family for BAFF-CD8a, Fusion Protein protein

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Product Notes

The BAFF-CD8a, Fusion Protein (Catalog #AAA634293) is a Protein produced from CHO cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's BAFF-CD8a, Fusion Protein can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Flow Cytometry (FC/FACS). Suitable for use in Flow Cytometry and ELISA. Researchers should empirically determine the suitability of the BAFF-CD8a, Fusion Protein for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BAFF-CD8a, Fusion Protein, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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