Mesothelin Active Protein | MPF active protein

Human Mesothelin

Cat. Num. AAA692300

• Gene Names: MSLN; MPF; SMRP
• Reactivity: Human
• Purity: > 95% by SDS-PAGE & HPLC analysis
• Synonyms: Mesothelin; Human Mesothelin; MPF; MSLN; SMRP; CAK1 antigen; ERC; Pre-pro-megakaryocyte-potentiating factor; MPF active protein

• Host: CHO cells
• Reactivity: Human
• Purity/Purification: > 95% by SDS-PAGE & HPLC analysis
• Form/Format: Lyophilized
• Sequence: EVEKTACPSG KKAREIDESL IFYKKWELEA CVDAALLATQ MDRVNA IPFT YEQLDVLKHK LDELYPQGYP ESVIQHLGYL FLKMSPEDIR K WNVTSLETL KALLEVNKGH EMSPQVATLI DRFVKGRGQL DKDTLDT LTA FYPGYLCSLS PEELSSVPPS SIWAVRPQDL DTCDPRQLDV LY PKARLAFQ NMNGSEYFVK IQSFLGGAPT EDLKALSQQN VSMDLATF MK LRTDAVLPLT VAEVQKLLGP HVEGLKAEER HRPVRDWILR QRQ DDLDTLG LGLQGGIPNG YLVLDLSMQE ALSGTPCLLG PGPVLTVLA L LLASTLA
• Sequence Length: 622

• Biological Activity: Determined by its ability to bind immobilized recombinant CA125/MUC16 in a functional ELISA.

Related Product Information for MPF active protein

Originally identified as a differentiation antigen of mesotheliomas, ovarian cystadenocarcinomas, and pancreatic adenocarcinomas, mesothelin is a glycosylphosphatidylinositol (GPI)-anchored, cell-surface glycoprotein predominantly secreted by cells of the mesothelium. Although mesothelin is expressed at restricted levels by normal mesothelial cells of the pleural, pericardial, and peritoneal membranes, aberrant expression has been documented in the aforementioned cancers, as well as in endometrioid uterine adenocarcinomas and squamous cell carcinomas of the esophagus, stomach, lung, and cervix. Proteolytic cleavage of mesothelin yields a soluble, polypeptide fragment-designated megakaryocyte-potentiating factor (MPF) based on its ability to stimulate megakaryocyte colony-forming activity of murine IL-3 in murine bone marrow cell cultures. Originally isolated from the HPC-Y5 pancreatic cell line, MPF has been suggested to play a role in the proliferation and differentiation of megakaryoctyes, and the regulation of resultant platelet production. While the biological functions of both mesothelin and MPF remain speculative, high levels of expression in cancerous tissues, compared to limited distribution in normal tissues, strongly suggests their involvement in tumorigenesis. Both have been demonstrated to promote tumor cell proliferation, migration, anchorage-independent growth, and tumor progression, demonstrating their involvement in heterotypic cell adhesion and the metastatic spread of cancer. Recombinant Human Mesothelin is a glycoprotein containing 327 amino acid residues, and has a calculated molecular weight of approximately 36.4 kDa. As a result of glycosylation, Recombinant Human Mesothelin migrates with an apparent molecular mass of approximately 40-45 kDa by SDS-PAGE gel, under reducing and non-reducing conditions.

Product Categories/Family for MPF active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 293651530
• NCBI GeneID: 10232
• NCBI Accession #: NP_001170826.1
• NCBI GenBank Nucleotide #: NM_001177355.2
• UniProt Accession #: Q13421; Q14859; Q4VQD5; Q96GR6; Q96KJ5; Q9BR17; Q9BTR2; Q9UCB2; Q9UK57; D3DU65
• Molecular Weight: 67,940 Da
• NCBI Official Full Name: mesothelin isoform 1 preproprotein
• NCBI Official Synonym Full Names: mesothelin
• NCBI Official Symbol: MSLN
• NCBI Official Synonym Symbols: MPF; SMRP
• NCBI Protein Information: mesothelin
• UniProt Protein Name: Mesothelin
• Protein Family: Mesothelin
• UniProt Gene Name: MSLN
• UniProt Synonym Gene Names: MPF; MPF

NCBI Description

This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]

Uniprot Description

MSLN: Membrane-anchored forms may play a role in cellular adhesion. Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer. Belongs to the mesothelin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: cell surface

Molecular Function: protein binding

Biological Process: cell-matrix adhesion