CD4 active protein

Human CD4, soluble

Cat. Num. AAA692480

• Gene Names: Cd4; L3T4; Ly-4
• Reactivity: Human
• Purity: > 95% by SDS-PAGE & HPLC analysis
• Synonyms: CD4; Human CD4; soluble; Cluster determinant 4; T-cell surface glycoprotein CD4; Interleukin IL-16 receptor; T-cell surface antigen T4/Leu-3; Leu-3(a; b); OKT4(a-f); L3T4 (mouse); CD4 active protein

• Host: CHO cells
• Reactivity: Human
• Purity/Purification: > 95% by SDS-PAGE & HPLC analysis
• Form/Format: Lyophilized
• Sequence: KKVVLGKKGD TVELTCTASQ KKSIQFHWKN SNQIKILGNQ GSFLTK GPSK LNDRADSRRS LWDQGNFPLI IKNLKIEDSD TYICEVEDQK E EVQLLVFGL TANSDTHLLQ GQSLTLTLES PPGSSPSVQC RSPRGKN IQG GKTLSVSQLE LQDSGTWTCT VLQNQKKVEF KIDIVVLAFQ KA SSIVYKKE GEQVEFSFPL AFTVEKLTGS GELWWQAERA SSSKSWIT FD LKNKEVSVKR VTQDPKLQMG KKLPLHLTLP QALPQYAGSG NLT LALEAKT GKLHQEVNLV VMRATQLQKN LTCEVWGPTS PKLMLSLKL E NKEAKVSKRE KAVWVLNPEA GMWQCLLSDS GQVLLESNIK VLPT WSTPVQ P
• Sequence Length: 457

• Biological Activity: Determined by its ability to binds HeLa cellss expressing cell-surface MHC-II molecules.

Related Product Information for CD4 active protein

Cluster determinant 4 (CD4), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, playsan integral role in signal transduction and T-cell differentiation, development and activation. CD4 is constitutively expressed on thesurface of various immune cells, including monocytes, macrophages, eosinophils, dendritic cells, and most prominently Tlymphocytes,where it functions as an essential co-receptor and co-ligand for T-cell receptor (TCR) and major histocompatibilitycomplex class II (MHC-II) molecules. Ligation by MHC-II molecules on the surface of antigen-presenting cells can serve toinfluence adaptive immunity by facilitating helper T-cell activation and macrophage differentiation, while ligation by proinflammatorycytokine IL-16 can contribute to innate immunity by chemoattracting CD4-expressing peripheral immune cells alongan IL-16 gradient for their recruitment and activation at sites of inflammation. The protean functionality of CD4 extends pastimmunity as CD4 also notably serves as the major receptor for HIV-1 and human herpes virus 7 (HHV-7) infections. During HIVpathogenesis, CD4 acts instrumentally as a high-affinity entry receptor for the internalization of HIV-1 following binding of the viralenvelope glycoprotein gp120 to CD4s extracellular domain. Recombinant Human sCD4 is amonomeric glycoprotein of 371 amino acid residues, which correspond to the extracellular CD4 domain, and a calculated molecularweight of 41.3 kDa. As a result of glycosylation, Recombinant Human sCD4 migrates with an apparent molecular mass of approximately 45-55 kDa by SDS-Page Gel analysis, under reducing conditions.

Product Categories/Family for CD4 active protein

Soluble receptors

NCBI and Uniprot Product Information

• NCBI GI #: 7304953
• NCBI GeneID: 12504
• NCBI Accession #: NP_038516.1
• NCBI GenBank Nucleotide #: NM_013488.2
• UniProt Accession #: P06332
• Molecular Weight: 24,473 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD4
• NCBI Official Synonym Full Names: CD4 antigen
• NCBI Official Symbol: Cd4
• NCBI Official Synonym Symbols: L3T4; Ly-4
• NCBI Protein Information: T-cell surface glycoprotein CD4
• UniProt Protein Name: T-cell surface glycoprotein CD4
• Protein Family: CD40 ligand
• UniProt Gene Name: Cd4

Uniprot Description

CD4: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI- CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6 F2|6 59.17 cM

Cellular Component: cell surface; endoplasmic reticulum lumen; endoplasmic reticulum membrane; external side of plasma membrane; lipid raft; plasma membrane

Molecular Function: enzyme binding; glycoprotein binding; immunoglobulin binding; MHC class II protein binding; protein homodimerization activity; protein kinase binding; zinc ion binding

Biological Process: cell surface receptor linked signal transduction; cytokine production; defense response to Gram-negative bacterium; induction by virus of cell-cell fusion in host; maintenance of cellular protein localization; positive regulation of calcium-mediated signaling; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein kinase activity; positive regulation of T cell activation; positive regulation of T cell proliferation; regulation of T cell activation; T cell activation; T cell differentiation; T cell selection