Angiopoietin-2 / Ang-2 Active Protein | Ang-2 active protein

Human Angiopoietin-2 / Ang-2

Cat. Num. AAA692183

• Gene Names: VCAN; WGN; ERVR; GHAP; PG-M; WGN1; CSPG2
• Reactivity: Human
• Purity: > 95%, by SDS-PAGE and HPLC analysis
• Synonyms: Angiopoietin-2 / Ang-2; Human Angiopoietin-2 / Ang-2; Recombinant Human Angiopoietin-2; Ang-2 active protein

• Host: CHO Cells
• Reactivity: Human
• Purity/Purification: > 95%, by SDS-PAGE and HPLC analysis
• Form/Format: Lyophilized
• Sequence: DAPLEYDDSV QRLQVLENIM ENNTQWLMKL ENYIQDNMKK EMVEIQ QNAV QNQTAVMIEI GTNLLNQTAE QTRKLTDVEA QVLNQTTRLE L QLLEHSLST NKLEKQILDQ TSEINKLQDK NSFLEKKVLA MEDKHII QLQ SIKEEKDQLQ VLVSKQNSII EELEKKIVTA TVNNSVLQKQ QH DLMETVNN LLTMMSTSNS AKDPTVAKEE QISFRDCAEV FKSGHTTN GI YTLTFPNSTE EIKAYCDMEA GGGGWTIIQR REDGSVDFQR TWK EYKVGFG NPSGEYWLGN EFVSQLTNQQ RYVLKIHLKD WEGNEAYSL Y EHFYLSSEEL NYRIHLKGLT GTAGKISSIS QPGNDFSTKD GDND KCICKC SQMLTGGWWF DACGPSNLNG MYYPQRQNTN KFNGIKWYYW KGSGYSLKAT TMMIRPADFH HHHHH
• Sequence Length: 655

• Endotoxin Level: < 0.1 ng per ug of Ang-2
• Buffer: 10 mM Sodium Phosphate, pH 8.0

Related Product Information for Ang-2 active protein

Human Angiopoitin-2 (Ang-2), a 66 kDa protein consisting of 476 amino acid residues (N21-F496), is fused to a N-terminal myc-tag and produced in insect cells. The angiopoietin (Ang) family of growth factors includes four members, all of which bind to the endothelial receptor tyrosine kinase Tie2. Two of the Angs, Ang-1 and Ang-4, activate the Tie2 receptor, whereas Ang-2 and Ang-3 inhibit Ang-1-induced Tie2 phosphorylation. Angiopoietin-1 (Ang-1) is a secreted growth factor which binds to and activates the Tie-2 receptor tyrosine kinase. The factor enhances endothelial cell survival and capillary morphogenesis, and also limits capillary permeability. Ang-2 binds the same receptor but fails to activate it: hence, it is a natural inhibitor of Ang-1. Ang-2 destabilizes capillary integrity, facilitating sprouting when ambient vascular endothelial growth factor (VEGF) levels are high, but causing vessel regression when VEGF levels are low. Tie-1 is a Tie-2 homologue but its ligands are unknown. Angiopoietin and Tie genes are expressed in the mammalian metanephros, the precursor of the adult kidney, where they may play a role in endothelial precursor growth. Tie-1-expressing cells can be detected in the metanephros when it first forms and, based on transplantation experiments, these precursors contribute to the generation of glomerular capillaries. During glomerular maturation, podocyte-derived Ang-1 and mesangial-cell-derived Ang-2 may affect growth of nascent capillaries. After birth, vasa rectae acquire their mature configuration and Ang-2 expressed by descending limbs of loops of Henle would be well placed to affect the growth of this medullary microcirculation. Finally, preliminary data implicate angiopoietins in deregulated vessel growth in Wilms\' kidney tumors and in vascular remodeling after nephrotoxicity. Altogether, existing data suggest that VEGF-A and Angiopoietins do not only have quite different roles during vascular development, but also very complementary and coordinated roles.

Product Categories/Family for Ang-2 active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 187607300
• NCBI GeneID: 1462
• NCBI Accession #: NP_001119808.1
• NCBI GenBank Nucleotide #: NM_001126336.2
• UniProt Accession #: Q15123; P20754; Q13010; Q13189; Q9UCL9; Q9UNW5; P13611
• Molecular Weight: 60-70 kDa
• NCBI Official Full Name: versican core protein isoform 2
• NCBI Official Synonym Full Names: versican
• NCBI Official Symbol: VCAN
• NCBI Official Synonym Symbols: WGN; ERVR; GHAP; PG-M; WGN1; CSPG2
• NCBI Protein Information: versican core protein; versican proteoglycan; large fibroblast proteoglycan; glial hyaluronate-binding protein; chondroitin sulfate proteoglycan 2; chondroitin sulfate proteoglycan core protein 2
• UniProt Protein Name: Versican core protein
• UniProt Gene Name: VCAN
• UniProt Synonym Gene Names: CSPG2; Chondroitin sulfate proteoglycan 2; GHAP
• UniProt Entry Name: CSPG2_HUMAN

NCBI Description

This gene is a member of the aggrecan/versican proteoglycan family. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and plays a central role in tissue morphogenesis and maintenance. Mutations in this gene are the cause of Wagner syndrome type 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]

Uniprot Description

Function: May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid.

Subunit structure: Interacts with FBLN1

By similarity.

Subcellular location: Secreted › extracellular space › extracellular matrix.

Tissue specificity: Cerebral white matter and plasma. Isoform V0 and isoform V1 are expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas. Isoform V2 is restricted to normal brain and gliomas. Isoform V3 is found in all these tissues except medulloblastomas. Ref.12

Developmental stage: Disappears after the cartilage development.

Post-translational modification: Phosphorylation sites are present in the extracellular medium.

Involvement in disease: Wagner vitreoretinopathy (WGVRP) [MIM:143200]: A rare vitreoretinopathy characterized by an optically empty vitreous cavity with fibrillary condensations and a preretinal avascular membrane. Other optical features include progressive chorioretinal atrophy, perivascular sheating, subcapsular cataract and myopia.Note: The disease is caused by mutations affecting the gene represented in this entry. The pathological mechanism involves a quantitave imbalance of the normally occurring splice variants (Ref.15). Ref.13 Ref.15

Sequence similarities: Belongs to the aggrecan/versican proteoglycan family.Contains 1 C-type lectin domain.Contains 2 EGF-like domains.Contains 1 Ig-like V-type (immunoglobulin-like) domain.Contains 2 Link domains.Contains 1 Sushi (CCP/SCR) domain.