CHO cells anti-Viral COVID 19 S1 Spike RBD Coronavirus Antibody | anti-COVID-19 antibody

Humanized Monoclonal Antibody against SARS-CoV-2 Spike Protein-1 Receptor-binding Domain (S1 RBD)

Cat. Num. AAA398091

• Reactivity: Viral
• Applications: ELISA
• Synonyms: COVID 19 S1 Spike RBD Coronavirus; Humanized Monoclonal Antibody against SARS-CoV-2 Spike Protein-1 Receptor-binding Domain (S1 RBD); 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; S1 Spike RBD; Receptor Binding Domain; anti-COVID-19 antibody

• Host: CHO cells
• Reactivity: Viral
• Isotype: Human IgG1 (humanized)
• Form/Format: Lyophilized from sterile PBS, pH 7.4.

• Applicable Applications for anti-COVID-19 antibody: ELISA (EIA)
• Application Notes: Optimal concentration/dilutions should be determined by end user.
• Antibody Note: Genetically engineered recombinant monoclonal IgG antibody
• Production: The cDNA encoding the highly variable region of the antibody recognizing S1RBD from mammalian cells were identified by phage display library, fused with the Fc fragment of human IgG1, and expressed in Chinese Hamster Ovary (CHO) cells.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Note: Centrifuge before opening to ensure the maximum recovery of vial contents.
• Preparation and Storage: Lyophilized protein can be stored at 2°C to 8°C for short-term (for < one week), and at -20°C to -80°C for long term. store. For reconstitution, add 100 ul of deionized water, mix gently and incubate the reconstituted product for 10 minutes at room temperature prior to use. Reconstituted antibody, if not being used immediately, should be aliquoted and kept at -20°C to -80°C. Avoid repeated freeze-thaw cycles.

Affinity Assay

(The binding between immobilized S1RBD (MBS398012) and the anti-SARS-CoV-2 S1RBD MBS398091.)

Related Product Information for anti-COVID-19 antibody

The SARS-CoV-2 glycosylated spike (S) protein highly exposed on the viral surface is a major determinant for virus binding and invasion into hose cells, which is a main target for neutralization antibody. The receptor-binding domain (RBD) in SARS-CoV-2 S protein is responsible for binding to human and bat angiotensin-converting enzyme 2 (ACE2) receptors.

References

1. Shajahan A, et al. (2020) Deducing the N- and O-glycosylation profile of the spike protein of novel coronavirus SARS-CoV-2. bioRxiv, https://doi.org/10.1101/2020.04.01.020966.
2. Walls, A C, et al. (2020) Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein. Cell, 181(2), 281-292.e6. https://doi.org/10.1016/j.cell.2020.02.058.