Mouse FABP5 Monoclonal Antibody | anti-FABP5 antibody

FABP5 (Marker of Metastatic Potential in Colorectal Cancer)

Cat. Num. AAA4380553

• Gene Names: FABP5; EFABP; KFABP; E-FABP; PAFABP; PA-FABP
• Reactivity: Human. Others not tested.
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: FABP5; Monoclonal Antibody; FABP5 (Marker of Metastatic Potential in Colorectal Cancer); E-FABP; EFABP; PA-FABP; PAFABP; anti-FABP5 antibody

• Host: Mouse
• Reactivity: Human. Others not tested.
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: CPTC-FABP5-3
• Specificity: This gene encodes the fatty acid binding protein found in epidermal cells, and was first identified as being upregulated in psoriasis tissue. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABPs may play roles in fatty acid uptake, transport, and metabolism. Polymorphisms in this gene are associated with type 2 diabetes. The human genome contains many pseudogenes similar to this locus. FABP5 is also associated with poor survival in triple-negative breast cancer. Additionally, FABP5 gene is upregulated in colorectal cancer cells compared to normal colon cells in a manner that correlateswith disease stage and that FABP5 significantly promotes colorectal cancercell growth and metastatic potential.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 135

• Applicable Applications for anti-FABP5 antibody: Western Blot (WB), Immunohistology (Formalin-Fixed)
• Application Notes: WB: 0.5-1.0ug/ml; Immunohistology (Formalin-Fixed): 0.5-1ug/ml for 30 min at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
• Immunogen: Recombinant humanfull length protein
• Positive Control: MOLT-4 and HEK-293 cell lines. Esophagus, Skin. Keratinocytes; highly expressed in psoriatic skin. Colon.
• Cellular Localization: Nucleus and cytoplasm.
• Chromosome Location: 8q21.13
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Esophagusstained with FABP5 Mouse Monoclonal Antibody (CPTC-FABP5-3).)

SDS-Page

(SDS-PAGE Analysis Purified FABP5 Mouse Monoclonal Antibody (CPTC-FABP5-3). Confirmation of Purity and Integrity of Antibody.)

Testing Data

(Analysis of Protein Array containing more than 19,000 full-length human proteins using FABP5 Mouse Monoclonal Antibody (CPTC-FABP5-3). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

References

Liu, Rong-Zong et al. The American Journal of Pathology 178.3 (2011): 997-100

NCBI and Uniprot Product Information

• NCBI GI #: 4557581
• NCBI GeneID: 2171
• NCBI Accession #: NP_001435.1
• NCBI GenBank Nucleotide #: NM_001444.2
• UniProt Accession #: Q01469; B2R4K0
• Molecular Weight: 15.4kDa
• NCBI Official Full Name: fatty acid-binding protein 5
• NCBI Official Synonym Full Names: fatty acid binding protein 5
• NCBI Official Symbol: FABP5
• NCBI Official Synonym Symbols: EFABP; KFABP; E-FABP; PAFABP; PA-FABP
• NCBI Protein Information: fatty acid-binding protein 5
• UniProt Protein Name: Fatty acid-binding protein 5
• Protein Family: Fatty acid-binding protein
• UniProt Gene Name: FABP5
• UniProt Synonym Gene Names: E-FABP1 PublicationManual assertion based on opinion iniRef.9; PA-FABP

NCBI Description

This gene encodes the fatty acid binding protein found in epidermal cells, and was first identified as being upregulated in psoriasis tissue. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABPs may play roles in fatty acid uptake, transport, and metabolism. Polymorphisms in this gene are associated with type 2 diabetes. The human genome contains many pseudogenes similar to this locus.[provided by RefSeq, Feb 2011]

Uniprot Description

Intracellular carrier for long-chain fatty acids and related active lipids, such as the endocannabinoid, that regulates the metabolism and actions of the ligands they bind. In addition to the cytosolic transport, selectively delivers specific fatty acids from the cytosol to the nucleus, wherein they activate nuclear receptors (PubMed:22170058). Delivers retinoic acid to the nuclear receptor peroxisome proliferator-activated receptor delta; which promotes proliferation and survival. May also serve as a synaptic carrier of endocannabinoid at central synapses and thus controls retrograde endocannabinoid signaling. Modulates inflammation by regulating PTGES induction via NF-kappa-B activation, and prostaglandin E2 (PGE2) biosynthesis during inflammation (). May be involved in keratinocyte differentiation (PubMed:8092987).