Irisin Active Protein | FNDC5 active protein

Irisin (human), (recombinant) (active)

Cat. Num. AAA565860

• Gene Names: FNDC5; FRCP2; irisin
• Applications: ELISA, Western Blot, SDS-Page
• Purity: >=90% (SDS-PAGE)
• Synonyms: Irisin; Irisin (human); (recombinant) (active); FNDC5 active protein

• Host: CHO cells
• Purity/Purification: >=90% (SDS-PAGE)
• Form/Format: Liquid. In 1X PBS

• Applicable Applications for FNDC5 active protein: ELISA (EIA), Western Blot (WB), SDS-PAGE, Activity assay
• Source Note: aa 32-143, also known as Irisin, comprises the majority of the extracellular domain of FNDC5. It is fused at the N-terminus to a DYKDDDDK-tag with an 8 aa linker between the DYKDDDDK-tag and Irisin
• Endotoxin: <0.01EU/ug
• Biological Activity: Treatment of 3T3L1 with 2ug/ml Irisin induces UCP-1 expression
• Preparation and Storage: Stable for up to a week when stored at 4 degree C and at least 6 months when stored at -80 degree C. Avoid freeze/thaw cycles; Shipping: Shipped on Dry Ice

Related Product Information for FNDC5 active protein

Irisin is a hormone that is involved in converting white adipose tissue into brown adipose tissue after physical exercise. It is formed after the membrane bound protein fibronectin type III domain containing protein 5 (FNDC5) is cleaved resulting in a secreted protein. FNDC5 is found in muscle tissue and is activated during physical exertion via the protein PGC1a. Soluble Irisin has also been shown to up regulate UCP-1 gene expression in mice. UCP-1 is a mitochondrial transporter protein and a marker of brown adipose tissue conversion. This suggests that Irisin plays an important role in energy regulation and furthermore may lead to improvements in glucose regulation, insulin sensitivity, hypertension, and obesity.

Product Categories/Family for FNDC5 active protein

Cellular Analysis; Metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 511093995
• NCBI GeneID: 252995
• NCBI Accession #: NP_001165411.2
• NCBI GenBank Nucleotide #: NM_001171940.1
• UniProt Accession #: Q8NAU1; Q6P6D9; Q7Z676; A6NMC9; D3DPQ6
• Molecular Weight: 15,527 Da
• NCBI Official Full Name: fibronectin type III domain-containing protein 5 isoform 3 preproprotein
• NCBI Official Synonym Full Names: fibronectin type III domain containing 5
• NCBI Official Symbol: FNDC5
• NCBI Official Synonym Symbols: FRCP2; irisin
• NCBI Protein Information: fibronectin type III domain-containing protein 5; fibronectin type III repeat-containing protein 2
• UniProt Protein Name: Fibronectin type III domain-containing protein 5
• Protein Family: Fibronectin type III domain-containing protein
• UniProt Gene Name: FNDC5
• UniProt Synonym Gene Names: FRCP2
• UniProt Entry Name: FNDC5_HUMAN

NCBI Description

This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Uniprot Description

FNDC5: Irisin: mediates beneficial effects of muscular exercise. Induces browning of white adipose tissue by stimulating UCP1 expression, at least in part, via the nuclear receptor PPARA. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p35.1

Cellular Component: peroxisomal membrane; endoplasmic reticulum; plasma membrane; integral to membrane; extracellular region

Molecular Function: hormone activity

Biological Process: response to muscle activity