Deoxyribonuclease I Active Protein

Deoxyribonuclease I, Recombinant, Human (DNase I)

Cat. Num. AAA637896

• Applications: Immunocytochemistry
• Synonyms: Deoxyribonuclease I; Recombinant; Human (DNase I); Deoxyribonuclease I active protein

• Host: CHO cells
• Form/Format: Supplied as a liquid in 1mM calcium chloride 150mM sodium chloride.

• Applicable Applications for Deoxyribonuclease I active protein: Immunocytochemistry (ICC)
• Specific Activity: 1000IU/1mg
• Molecular Formula: C1321H1995O396S9
• Unit Definition: Dnase is generally assayed according to the photometric method developed by Kunitz (see Kunitz, M., Journal of General Physiology, vol 33, p.349 and 363, 1950). One Dnase unit results in an increase in absorbance at 260nm of 0.001/minute at 25 degree C when acting upon highly polymerized solution of DNA at pH-5. Also 0.005 Kunitz unit digests 1ug of lambda phage DNA in 10 minutes at 37 degree C in 50mM Tris, 1mMMg++, pH 7.8 in a 50ul reaction.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20 degree C. Aliquots are stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for Deoxyribonuclease I active protein

Recombinant Human Deoxyribonuclease I (rhDNase), an enzyme which selectively cleaves DNA. Recombinant Human Dnase is an endonuclease enzyme which splits phosphodiester linkages within polynucleotides, acting primarily on single stranded DNA (ssDNA), double stranded DNA (ddDNA) and chromatin. Dnase is activated by bivalent metals such as Mg+2 and Ca+2. Dnase enzymes are common reagents used in biochemical methods requiring diestion of DNA and recovery of RNA, or where DNA is to be removed without affecting structural proteins or enzymes. Dnase enzymes are also used in tissue culture to digest DNA from damaged cells, resulting in reduced viscosity, and for removal of membrane-bound DNA fragments. Recombinant Human DNase produced in CHO is a glycosylated, polypeptide chain containing 260aa and having a total molecular mass of 37kD with a molecular formula of C1321H1995O396S9.

Product Categories/Family for Deoxyribonuclease I active protein

Cloning; Cloning-Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 1197173
• Molecular Weight: 37kD
• NCBI Official Full Name: deoxyribonuclease I

Uniprot Description

DNASE1: Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. Defects in DNASE1 are a cause of susceptibility to systemic lupus erythematosus (SLE). A chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Belongs to the DNase I family.

Protein type: Deoxyribonuclease; Secreted, signal peptide; EC 3.1.21.1; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: extracellular region; nuclear envelope; nucleus

Molecular Function: protein binding; deoxyribonuclease I activity; actin binding; endodeoxyribonuclease activity

Biological Process: apoptosis; DNA catabolic process, endonucleolytic

Disease: Systemic Lupus Erythematosus