Chromatin Modifying Protein 1A Recombinant Protein | CHMP1A recombinant protein

Recombinant Human Chromatin Modifying Protein 1A

Cat. Num. AAA146022

• Gene Names: CHMP1A; PCH8; CHMP1; PRSM1; PCOLN3; VPS46A; VPS46-1
• Purity: Greater than 85.0% as determined by SDS-PAGE.
• Synonyms: Chromatin Modifying Protein 1A; Recombinant Human Chromatin Modifying Protein 1A; CHMP1A Human; Chromatin Modifying Protein 1A Human Recombinant; Charged multivesicular body protein 1a; Chromatin-modifying protein 1a; Vacuolar protein sorting-associated protein 46-1; Vps46-1; hVps46-1; CHMP1A; CHMP1; KIAA0047; PCOLN3; PRSM1; PCH8; VPS46A; CHMP1A recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 85.0% as determined by SDS-PAGE.
• Form/Format: CHMP1A protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 20% glycerol and 1mM DTT.; Sterile Filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMDDTLFQ LKFTAKQLEK LAKKAEKDSK AEQAKVKKAL LQKNVECARV YAENAIRKKN EGVNWLRMAS RVDAVASKVQ TAVTMKGVTK NMAQVTKALD KALSTMDLQK VSSVMDRFEQ QVQNLDVHTS VMEDSMSSAT TLTTPQEQVD SLIMQIAEEN GLEVLDQLSQ LPEGASAVGE SSVRSQEDQL SRRLAALRN.
• Sequence Length: 196

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for CHMP1A recombinant protein

Description: CHMP1A Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 219 amino acids (1-196 a.a.) and having a molecular mass of 24.1kDa (Molecular size on SDS-PAGE will appear higher).CHMP1A is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: Charged multivesicular body protein 1a (CHMP1A) is a member of the vacuolar sorting protein family and functions as chromatin-modifying protein. These complexes are essential for sorting endosomal articles into multivesicular bodies (MVBs), as well as vital for the formation of these bodies. The MVBs pathway mediates distribution of transmembrane proteins into the lumen of the lysosome for degradation. CHMP1 interacts with VPS4B and localizes to early endosomes.

Product Categories/Family for CHMP1A recombinant protein

Recombinant & Natural Proteins; Recombinant Proteins; Chromatin Modifying Protein

NCBI and Uniprot Product Information

• NCBI GI #: 103485496
• NCBI GeneID: 5119
• NCBI Accession #: NP_002759.2
• NCBI GenBank Nucleotide #: NM_002768.4
• UniProt Accession #: Q9HD42; Q14468; Q15779; Q96G31; A2RU09
• Molecular Weight: 7,363 Da
• NCBI Official Full Name: charged multivesicular body protein 1a isoform 2
• NCBI Official Synonym Full Names: charged multivesicular body protein 1A
• NCBI Official Symbol: CHMP1A
• NCBI Official Synonym Symbols: PCH8; CHMP1; PRSM1; PCOLN3; VPS46A; VPS46-1
• NCBI Protein Information: charged multivesicular body protein 1a; charged multivesicular body protein 1/chromatin modifying protein 1; chromatin modifying protein 1A; procollagen (type III) N-endopeptidase; protease, metallo, 1, 33kD; vacuolar protein sorting-associated protein 46-1
• UniProt Protein Name: Charged multivesicular body protein 1a
• Protein Family: Charged multivesicular body protein
• UniProt Gene Name: CHMP1A
• UniProt Synonym Gene Names: CHMP1a; Vps46-1; hVps46-1
• UniProt Entry Name: CHM1A_HUMAN

NCBI Description

This gene encodes a member of the CHMP/Chmp family of proteins which are involved in multivesicular body sorting of proteins to the interiors of lysosomes. The initial prediction of the protein sequence encoded by this gene suggested that the encoded protein was a metallopeptidase. The nomenclature has been updated recently to reflect the correct biological function of this encoded protein. Several transcripts encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012]

Uniprot Description

CHMP1A: Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT- III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in cytokinesis. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. May also be involved in chromosome condensation. Targets the Polycomb group (PcG) protein BMI1/PCGF4 to regions of condensed chromatin. May play a role in stable cell cycle progression and in PcG gene silencing. Belongs to the SNF7 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Protease

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: condensed nuclear chromosome; nuclear matrix; early endosome; endomembrane system; microtubule organizing center

Molecular Function: protein domain specific binding; protein binding; protein homodimerization activity; zinc ion binding; metallopeptidase activity

Biological Process: vesicle-mediated transport; protein transport; cell separation during cytokinesis; transcription, DNA-dependent; negative regulation of transcription by glucose; mitotic chromosome condensation; vacuolar transport; cytokinesis; gene silencing; negative regulation of transcription, DNA-dependent; proteolysis; mitotic metaphase plate congression; nuclear organization and biogenesis

Disease: Pontocerebellar Hypoplasia, Type 8