Typical Testing Data/Standard Curve (for reference only)
Chicken Inositol monophosphatase 1 (IMPA1) ELISA Kit | IMPA1 elisa kit
Chicken Inositol monophosphatase 1 (IMPA1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: IMPA1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IMPA1 antibody and an IMPA1-HRP conjugate. The assay sample and buffer are incubated together with IMPA1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IMPA1 concentration since IMPA1 from samples and IMPA1-HRP conjugate compete for the anti-IMPA1 antibody binding site. Since the number of sites is limited, as more sites are occupied by IMPA1 from the sample, fewer sites are left to bind IMPA1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IMPA1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an enyzme that dephosphorylates myo-inositol monophosphate to generate free myo-inositol, a precursor of phosphatidylinositol, and is therefore an important modulator of intracellular signal transduction via the production of the second messengers myoinositol 1,4,5-trisphosphate and diacylglycerol. This enzyme can also use myo-inositol-1,3-diphosphate, myo-inositol-1,4-diphosphate, scyllo-inositol-phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates. This enzyme shows magnesium-dependent phosphatase activity and is inhibited by therapeutic concentrations of lithium. Inhibition of inositol monophosphate hydroylosis and subsequent depletion of inositol for phosphatidylinositol synthesis may explain the anti-manic and anti-depressive effects of lithium administered to treat bipolar disorder. Alternative splicing results in multiple transcript variants encoding distinct isoforms. A pseudogene of this gene is also present on chromosome 8q21.13. [provided by RefSeq, Nov 2009]
Uniprot Description
IMPA1: Responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides and has been implicated as the pharmacological target for lithium action in brain. Can use myo-inositol monophosphates, myo-inositol 1,3-diphosphate, myo-inositol 1,4-diphosphate, scyllo-inositol- phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-1- phosphate, beta-glycerophosphate, and 2'-AMP as substrates. Belongs to the inositol monophosphatase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.1.3.25; Phosphatase (non-protein); Carbohydrate Metabolism - inositol phosphate; Motility/polarity/chemotaxis; EC 3.1.3.94
Chromosomal Location of Human Ortholog: 8q21.13-q21.3
Cellular Component: cytoplasm; cytosol
Molecular Function: identical protein binding; protein homodimerization activity; manganese ion binding; inositol-1(or 4)-monophosphatase activity; magnesium ion binding; lithium ion binding
Biological Process: inositol phosphate metabolic process; phosphoinositide phosphorylation; inositol phosphate dephosphorylation; inositol biosynthetic process; phosphatidylinositol biosynthetic process; signal transduction; phosphate metabolic process
Research Articles on IMPA1
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Product Notes
The Chicken IMPA1 impa1 (Catalog #AAA7235623) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7235623 ELISA Kit recognizes Chicken IMPA1. It is sometimes possible for the material contained within the vial of "Inositol monophosphatase 1 (IMPA1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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