Chicken IGF1R ELISA Kit | IGF1R elisa kit

Chicken IGF1R (Insulin Like Growth Factor 1 Receptor) ELISA Kit

Cat. Num. AAA2500691

• Gene Names: IGF1R; IGFR; CD221; IGFIR; JTK13
• Reactivity: Chicken
• Synonyms: IGF1R; Chicken IGF1R (Insulin Like Growth Factor 1 Receptor) ELISA Kit; IGF1R elisa kit

• Reactivity: Chicken
• Specificity: This kit recognizes natural and recombinant Chicken IGF1R. No significant cross-reactivity or interference between Chicken IGF1R and analogues was observed.
• Sequence Length: 1367

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.313-20ng/mL
• Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL
• Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Chicken IGF1R concentrations in serum, plasma and other biological fluids.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IGF1R elisa kit

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IGF1R. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IGF1R and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IGF1R, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IGF1R. You can calculate the concentration of IGF1R in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557665
• NCBI GeneID: 3480
• NCBI Accession #: NP_000866.1
• NCBI GenBank Nucleotide #: NM_000875.4
• UniProt Accession #: P08069; Q14CV2; Q9UCC0; B1B5Y2
• Molecular Weight: 154,793 Da
• NCBI Official Full Name: insulin-like growth factor 1 receptor isoform 1
• NCBI Official Synonym Full Names: insulin-like growth factor 1 receptor
• NCBI Official Symbol: IGF1R
• NCBI Official Synonym Symbols: IGFR; CD221; IGFIR; JTK13
• NCBI Protein Information: insulin-like growth factor 1 receptor; IGF-I receptor; insulin-like growth factor I receptor; soluble IGF1R variant 1; soluble IGF1R variant 2
• UniProt Protein Name: Insulin-like growth factor 1 receptor
• Protein Family: Insulin-like growth factor 1 receptor
• UniProt Gene Name: IGF1R
• UniProt Synonym Gene Names: IGF-I receptor
• UniProt Entry Name: IGF1R_HUMAN

NCBI Description

This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]

Uniprot Description

IGF1R: a receptor tyrosine kinase that binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. Functions as an anti-apoptotic agent by enhancing cell survival. Can play a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand- binding domain, while the beta chain carries the kinase domain. Interacts with PIK3R1 and with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues. Mutated in rare cases of pre- and post-natal growth retardation. One SNP associated with increased human longevity. Increased expression of IGF1R and other pathway members associated with progression and malignancy in a range of cancers. Inhibitors: AG1024, AEW541.

Protein type: Protein kinase, TK; EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Protein kinase, tyrosine (receptor); TK group; InsR family

Chromosomal Location of Human Ortholog: 15q26.3

Cellular Component: neuron projection; membrane; intracellular membrane-bound organelle; integral to plasma membrane; plasma membrane; caveola; receptor complex

Molecular Function: insulin binding; identical protein binding; insulin-like growth factor binding; protein binding; insulin-like growth factor I binding; protein-tyrosine kinase activity; insulin receptor substrate binding; phosphoinositide 3-kinase binding; G-protein alpha-subunit binding; ATP binding; insulin receptor binding; insulin-like growth factor receptor activity

Biological Process: phosphoinositide 3-kinase cascade; epidermis development; inactivation of MAPKK activity; protein heterooligomerization; protein amino acid autophosphorylation; exocrine pancreas development; signal transduction; regulation of JNK cascade; response to vitamin E; positive regulation of MAPKKK cascade; mammary gland development; male sex determination; positive regulation of cell proliferation; protein tetramerization; positive regulation of cytokinesis; negative regulation of protein kinase B signaling cascade; phosphoinositide-mediated signaling; positive regulation of mitosis; negative regulation of transcription factor activity; positive regulation of protein kinase B signaling cascade; axonogenesis; insulin-like growth factor receptor signaling pathway; establishment of cell polarity; insulin receptor signaling pathway; immune response; brain development; positive regulation of DNA replication; negative regulation of apoptosis; positive regulation of cell migration

Disease: Insulin-like Growth Factor I, Resistance To