Typical Testing Data/Standard Curve (for reference only)
Chicken HSP70 (Heat Shock Protein 70 high senstivity) ELISA Kit | HSP70 elisa kit
Chicken HSP70 (Heat Shock Protein 70 high senstivity)
Reactivity
Chicken
Synonyms
HSP70 (Heat Shock Protein 70 high senstivity); Chicken HSP70 (Heat Shock Protein 70 high senstivity); DAAP-21F2.7; FLJ54328; HSP70-1B; HSP70-2; HSPA1A; heat shock 70kD protein 1B; HSP70 elisa kit
Reactivity
Chicken
Specificity
This assay has high sensitivity and excellent specificity for detection of HSP70 . No significant cross-reactivity or interference between HSP70 and analogues was observed.
Sequence Length
629
Samples
Serum, plasma and other biological fluids.
Assay Type
Sandwich ELISA, Double Antibody
Detection Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HSP70 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HSP70 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
4 degree C for 6 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for HSP70 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- HSP70 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-HSP70 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the HSP70 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of HSP70 can be calculated.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
68,852 Da
NCBI Official Full Name
HSP70, partial
UniProt Protein Name
HSP70
Protein Family
UniProt Gene Name
HSP70
UniProt Entry Name
A0A0H3WA26_CRYHO
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Product Notes
The Chicken HSP70 hsp70 (Catalog #AAA766069) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA766069 ELISA Kit recognizes Chicken HSP70. It is sometimes possible for the material contained within the vial of "HSP70 (Heat Shock Protein 70 high senstivity), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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