Chicken High mobility group protein B1 (HMGB1) ELISA Kit | HMGB1 elisa kit

Chicken High mobility group protein B1 (HMGB1) ELISA Kit

Cat. Num. AAA287094

• Gene Names: HMGB3; HMG4; HMG-1; HMG2a; HMGB1
• Reactivity: Chicken
• Synonyms: High mobility group protein B1 (HMGB1); Chicken High mobility group protein B1 (HMGB1) ELISA Kit; DKFZp686A04236; HMG1; HMG3; SBP-1; Amphoterin; OTTHUMP00000018196; OTTHUMP00000190860; OTTHUMP00000200117; Sulfoglucuronyl carbohydrate binding protein; high mobility group box 1; high mobility group prot; HMGB1 elisa kit

• Reactivity: Chicken
• Specificity: This assay has high sensitivity and excellent specificity for detection of Chicken HMGB1. No significant cross-reactivity or interference between Chicken HMGB1 and analogues was observed.
• Sequence Length: 217

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for HMGB1 elisa kit

This assay employs a two-site sandwich ELISA to quantitate HMGB1 in samples. An antibody specific for HMGB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HMGB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HMGB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HMGB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 347921933
• NCBI GeneID: 396232
• NCBI Accession #: NP_990626.2
• NCBI GenBank Nucleotide #: NM_205295.1
• UniProt Accession #: P36194; F1NGP8; P40618
• Molecular Weight: 23,057 Da
• NCBI Official Full Name: high mobility group protein B3
• NCBI Official Synonym Full Names: high mobility group box 3
• NCBI Official Symbol: HMGB3
• NCBI Official Synonym Symbols: HMG4; HMG-1; HMG2a; HMGB1
• NCBI Protein Information: high mobility group protein B3
• UniProt Protein Name: High mobility group protein B3
• Protein Family: High mobility group protein
• UniProt Gene Name: HMGB3
• UniProt Synonym Gene Names: HMG2A; HMG4; HMG-2a; HMG-4

Uniprot Description

Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. Associates with chromatin and binds DNA with a preference to non-canonical DNA structures such as single-stranded DNA. Can bent DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters (). Binds to the delta-1 crystallin/ASL1 enhancer (PubMed:7904558). Proposed to be involved in the innate immune response to nucleic acids by acting as a cytoplasmic promiscuous immunogenic DNA/RNA sensor ().