Chicken CD28 ELISA Kit | CD28 elisa kit

Chicken CD28 (Cluster of Differentiation 28) ELISA Kit

Cat. Num. AAA2505627

• Reactivity: Chicken
• Synonyms: CD28; Chicken CD28 (Cluster of Differentiation 28) ELISA Kit; CD28 elisa kit

• Reactivity: Chicken
• Specificity: This kit recognizes natural and recombinant Chicken CD28. No significant cross-reactivity or interference between Chicken CD28 and analogues was observed.
• Sequence Length: 63

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 3.125-200ng/mL
• Sensitivity: Min: 1.875ng/mL; Max: 200ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CD28 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Chicken CD28 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CD28. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CD28 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CD28, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CD28. You can calculate the concentration of CD28 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 192485
• NCBI GeneID: 12487
• UniProt Accession #: P31041; Q6GSH7
• Molecular Weight: 25,243 Da
• NCBI Official Full Name: CD28, partial
• NCBI Official Synonym Full Names: CD28 antigen
• NCBI Official Symbol: Cd28
• NCBI Protein Information: T-cell-specific surface glycoprotein CD28
• UniProt Protein Name: T-cell-specific surface glycoprotein CD28
• Protein Family: T-cell-specific surface glycoprotein
• UniProt Gene Name: Cd28
• UniProt Entry Name: CD28_MOUSE

Uniprot Description

CD28: a type I plasma membrane protein Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Binds to B7-1 and -2. Expressed in T cells and plasma cells, but not in less mature B cells. Six splice-variant isoforms have been described.

Protein type: Membrane protein, integral; Cell surface

Cellular Component: membrane; plasma membrane; integral to membrane; immunological synapse; external side of plasma membrane; lipid raft

Molecular Function: protein homodimerization activity; SH3/SH2 adaptor activity; protease binding

Biological Process: positive regulation of isotype switching to IgG isotypes; T cell activation; positive regulation of mitosis; positive regulation of interleukin-2 biosynthetic process; T cell receptor signaling pathway; negative thymic T cell selection; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of inflammatory response to antigenic stimulus; positive regulation of protein kinase B signaling cascade; T cell costimulation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of T cell proliferation; immune response; positive regulation of alpha-beta T cell proliferation; regulatory T cell differentiation