Typical Testing Data/Standard Curve (for reference only)
Chicken BGN ELISA Kit | BGN elisa kit
Chicken BGN (Biglycan) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to BGN. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for BGN and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain BGN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of BGN. You can calculate the concentration of BGN in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a small cellular or pericellular matrix proteoglycan that is closely related in structure to two other small proteoglycans, decorin and fibromodulin. The encoded protein and decorin are thought to be the result of a gene duplication. Decorin contains one attached glycosaminoglycan chain, while this protein probably contains two chains. For this reason, this protein is called biglycan. This protein plays a role in assembly of collagen fibrils and muscle regeneration. It interacts with several proteins involved in muscular dystrophy, including alpha-dystroglycan, alpha- and gamma-sarcoglycan and collagen VI, and it is critical for the assembly of the dystrophin-associated protein complex. [provided by RefSeq, Nov 2009]
Uniprot Description
BGN: May be involved in collagen fiber assembly. Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: Xq28
Cellular Component: extracellular matrix; lysosomal lumen; proteinaceous extracellular matrix; transport vesicle; cell surface; Golgi lumen; extracellular region; sarcolemma
Molecular Function: glycosaminoglycan binding; extracellular matrix binding; extracellular matrix structural constituent
Biological Process: chondroitin sulfate metabolic process; extracellular matrix organization and biogenesis; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; chondroitin sulfate catabolic process; carbohydrate metabolic process; pathogenesis; blood vessel remodeling; peptide cross-linking via chondroitin 4-sulfate glycosaminoglycan; dermatan sulfate biosynthetic process
Research Articles on BGN
Similar Products
Product Notes
The Chicken BGN bgn (Catalog #AAA2509930) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2509930 ELISA Kit recognizes Chicken BGN. It is sometimes possible for the material contained within the vial of "BGN, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
