Chicken Aminopeptidase N (ANPEP) ELISA Kit | ANPEP elisa kit

Chicken Aminopeptidase N (ANPEP) ELISA Kit

Cat. Num. AAA287833

• Gene Names: ANPEP; APDE; CD13; p150
• Reactivity: Chicken
• Synonyms: Aminopeptidase N (ANPEP); Chicken Aminopeptidase N (ANPEP) ELISA Kit; APN; CD13; GP150; LAP1; P150; PEPN; aminopeptidase M; aminopeptidase N; membrane alanine aminopeptidase; microsomal aminopeptidase; myeloid plasma membrane glycoprotein CD13; ANPEP elisa kit

• Reactivity: Chicken
• Specificity: This assay has high sensitivity and excellent specificity for detection of Chicken ANPEP. No significant cross-reactivity or interference between Chicken ANPEP and analogues was observed.
• Sequence Length: 967

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for ANPEP elisa kit

This assay employs a two-site sandwich ELISA to quantitate ANPEP in samples. An antibody specific for ANPEP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANPEP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANPEP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANPEP bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 45382361
• NCBI GeneID: 395667
• NCBI Accession #: NP_990192.1
• NCBI GenBank Nucleotide #: NM_204861.1
• UniProt Accession #: O57579; A0A1D5NWZ2; D2KKL5
• Molecular Weight: 109,132 Da
• NCBI Official Full Name: aminopeptidase Ey
• NCBI Official Synonym Full Names: alanyl aminopeptidase, membrane
• NCBI Official Symbol: ANPEP
• NCBI Official Synonym Symbols: APDE; CD13; p150
• NCBI Protein Information: aminopeptidase Ey; aminopeptidase N
• UniProt Protein Name: Aminopeptidase Ey
• Protein Family: Aminopeptidase
• UniProt Gene Name: ANPEP
• UniProt Synonym Gene Names: APDE

Uniprot Description

Broad specificity aminopeptidase. Degrades a variety of peptides possessing various N-terminal amino acids including hydrophobic, basic and acidic amino acids. Preferentially hydrolyzes small peptides consisting of 4 or 5 amino acids. Hydrolyzes the N-terminal Xaa-Pro bonds in the chicken brain peptide Leu-Pro-Leu-Arg-PheNH2, the substance P fragment Arg-Pro-Lys-Pro and the bradykinin fragment Arg-Pro-Pro-Gly-Phe. Hydrolyzes the N-formylated peptides fMet-Leu-Phe, fMet-Ala-Gly-Ser-Glu and fMet-Nle-Leu-Phe-Nle-Tyr-Lys, but does not hydrolyze peptides with acetylation or pyroglutamic acid at N-terminus. Does not hydrolyze large peptides such as complete substance P, bradykinin or schistoFLRFamide.