Chicken Adipocyte fatty-acid binding protein (aFABP) ELISA Kit | aFABP elisa kit

Chicken Adipocyte fatty-acid binding protein (aFABP) ELISA Kit

Cat. Num. AAA262923

• Gene Names: Fabp4; aP2; Albp
• Reactivity: Chicken
• Synonyms: Adipocyte fatty-acid binding protein (aFABP); Chicken Adipocyte fatty-acid binding protein (aFABP) ELISA Kit; aFABP elisa kit

• Reactivity: Chicken
• Specificity: No cross-reaction with other factors.
• Sequence Length: 132

• Samples: Chicken serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 2000 pg/ml-31.2 pg/ml
• Sensitivity: Up to 12 pg/ml
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for aFABP elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Chicken aFABP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 1658525
• NCBI GeneID: 79451
• Molecular Weight: 14,708 Da
• NCBI Official Full Name: adipocyte fatty acid binding protein
• NCBI Official Synonym Full Names: fatty acid binding protein 4, adipocyte
• NCBI Official Symbol: Fabp4
• NCBI Official Synonym Symbols: aP2; Albp
• NCBI Protein Information: fatty acid-binding protein, adipocyte; AFABP; A-FABP; fatty acid-binding protein 4; adipocyte lipid-binding protein; adipocyte-type fatty acid-binding protein
• UniProt Protein Name: Fatty acid-binding protein, adipocyte
• UniProt Gene Name: Fabp4
• UniProt Synonym Gene Names: ALBP; A-FABP; AFABP
• UniProt Entry Name: FABP4_RAT

NCBI Description

intracellular lipid-binding protein; mediates fatty acid trafficking during lipolysis [RGD, Feb 2006]

Uniprot Description

FABP4: a lipid transport protein that is the predominant fatty acid binding protein found in adipocytes. Also expressed in macrophages. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus. Homodimer. Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. Interacts with PPARG. Monomer. FABP4 knockout mice fed a high-fat and high-calorie diet become obese but develop neither insulin resistance nor diabetes, suggesting that this protein might be a link between obesity and insulin resistance and diabetes. Mice deficient in both FABP4 and ApoE show protection against atherosclerosis when compared with mice deficient only in ApoE. Mice carrying a FABP4 genetic variant exhibit both reduced FABP4 expression and a reduced potential for developing type 2 diabetes and coronary heart disease. A related study in humans indicated a similar pattern, suggesting that FABP4 may be a potential therapeutic target in the treatment of these disorders. Belongs to the calycin superfamily,fatty-acid binding protein (FABP) family.

Protein type: Lipid-binding

Cellular Component: cytoplasm; lipid particle; cytosol; nucleus

Molecular Function: protein binding; transporter activity; fatty acid binding; lipid binding

Biological Process: response to drug; cholesterol homeostasis; transport; response to glucocorticoid stimulus; positive regulation of cell proliferation; white fat cell differentiation; brown fat cell differentiation; negative regulation of protein kinase activity; cytokine production; negative regulation of transcription, DNA-dependent; fatty acid metabolic process; positive regulation of inflammatory response