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Chicken Lysozyme (LZM) DIY ELISA Kit | LZM diy elisa kit

Chicken Lysozyme (LZM) ELISA Kit DIY Materials

Gene Names
LYZ; LZM; LYZF1
Reactivity
Chicken
Synonyms
Lysozyme (LZM); Chicken Lysozyme (LZM) ELISA Kit DIY Materials; LYZ; Renal Amyloidosis; N-Acetylmuramide Glycanhydrolase; Muramidase; 1; 4-beta-N-acetylmuramidase C; LZM diy elisa kit
Ordering
For Research Use Only!
Reactivity
Chicken
Specificity
The Abs in the kit have high sensitivity and excellent specificity for detection of Lysozyme (LZM). No significant cross-reactivity or interference between Lysozyme (LZM) and analogues was observed.
Sequence Length
148
Application Notes
Main materials for "Do It Yourself ELISA Kit"
Assay Type
Double-antibody Sandwich ELISA for Antigen Detection
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.625-40ng/mL
Sensitivity
0.262ng/mL
Reagent Contents
Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Product Usage
1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Other Reagents Required
ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
Preparation and Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.
Product Categories/Family for LZM diy elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
16,537 Da
NCBI Official Full Name
lysozyme (EC 3.2.1.17)
NCBI Official Synonym Full Names
lysozyme
NCBI Official Symbol
LYZ
NCBI Official Synonym Symbols
LZM; LYZF1
NCBI Protein Information
lysozyme C
UniProt Protein Name
Lysozyme C
UniProt Gene Name
LYZ
UniProt Synonym Gene Names
LZM

NCBI Description

This gene encodes human lysozyme, whose natural substrate is the bacterial cell wall peptidoglycan (cleaving the beta[1-4]glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the antimicrobial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. The protein has antibacterial activity against a number of bacterial species. Missense mutations in this gene have been identified in heritable renal amyloidosis. [provided by RefSeq, Oct 2014]

Uniprot Description

LYZ: Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte- macrophage system and enhance the activity of immunoagents. Defects in LYZ are a cause of amyloidosis type 8 (AMYL8); also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. Belongs to the glycosyl hydrolase 22 family.

Protein type: EC 3.2.1.17; Endoplasmic reticulum; Hydrolase; Secreted; Secreted, signal peptide; Vesicle

Chromosomal Location of Human Ortholog: 12q15

Cellular Component: extracellular region; extracellular space

Molecular Function: identical protein binding; lysozyme activity

Biological Process: cell wall catabolic process; cellular protein metabolic process; defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; inflammatory response; retinal homeostasis

Disease: Amyloidosis, Familial Visceral

Research Articles on LZM

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Product Notes

The Chicken LZM lyz (Catalog #AAA2089035) is a DIY ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2089035 DIY ELISA Kit recognizes Chicken LZM. Main materials for "Do It Yourself ELISA Kit". Researchers should empirically determine the suitability of the LZM lyz for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lysozyme (LZM), DIY ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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