Chicken Botulinum Neurotoxin Type B Antibody | anti-botB antibody

Chicken anti Botulinum Neurotoxin Type B

Cat. Num. AAA396868

• Purity: Affinity Purified
• Synonyms: Botulinum Neurotoxin Type B; Chicken anti Botulinum Neurotoxin Type B; anti-botB antibody

• Host: Chicken
• Purity/Purification: Affinity Purified
• Form/Format: Liquid; 0.05 mL, Phosphate Buffered Saline‚ pH 7.2 with 0.075% sodium azide
• Concentration: 2.0 mg/mL (varies by lot)
• Sequence Length: 1291

• Source Note: Chickens were immunized with highly purified Botulinum Neurotoxin Type B (Clostridium botulinum). After multiple immunizations in Freund's adjuvant, eggs were collected and the IgY purified. Anti-Botulinum Neurotoxin Type B was immunoaffinity purified using the immunogen immobilized on a solid support.
• Preparation and Storage: Store at 4 degree C.

Product Categories/Family for anti-botB antibody

Primary Antibodies

References

1. Zhang Y., Lou J., Jenko K.L., Marks, J.D. and S.M. Varnum. 2012. Simultaneous and sensitive detection of six serotypes of botulinum neurotoxin using enzyme-linked immunosorbent assay-based protein antibody microarrays. Anal. Biochem. 1430(2):185-92.

NCBI and Uniprot Product Information

• NCBI GI #: 399134
• UniProt Accession #: P10844; P10843
• Molecular Weight: 150,803 Da
• NCBI Official Full Name: Botulinum neurotoxin type B
• UniProt Protein Name: Botulinum neurotoxin type B
• Protein Family: Botulinum neurotoxin
• UniProt Gene Name: botB
• UniProt Synonym Gene Names: LC; HC

Uniprot Description

Botulinum neurotoxin type B: Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of the eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure. Precursor of botulinum neurotoxin B which has 2 coreceptors; complex polysialylated gangliosides found on neural tissue and specific membrane-anchored proteins found in synaptic vesicles (PubMed:17185412, PubMed:17167421, PubMed:17167418, PubMed:23807078). Receptor proteins are exposed on host presynaptic cell membrane during neurotransmitter release, when the toxin heavy chain (HC) binds to them (PubMed:14504267). Upon synaptic vesicle recycling the toxin is taken up via the endocytic pathway (PubMed:14504267). When the pH of the toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the HC forms pores that allows the light chain (LC) to translocate into the cytosol (PubMed:3856850). Once in the cytosol the disulfide bond linking the 2 subunits is reduced and LC cleaves its target protein on synaptic vesicles, preventing their fusion with the cytoplasmic membrane and thus neurotransmitter release. Binds to host peripheral neuronal presynaptic membranes via synaptotagmins 1 and 2 (SYT1 and SYT2) (PubMed:8144634, PubMed:14504267). Toxin binds to the membrane proximal extra-cytoplasmic region of host SYT1 and SYT2 that is transiently exposed outside of cells during exocytosis; exogenous gangliosides enhance binding and subsequent uptake of toxin into host cells (PubMed:14504267, PubMed:15123599). Toxin uptake into neural cells requires stimulation (incubation with K+ to stimulate SYT protein receptor exposure); subsequently the toxin colocalizes with its receptor in host cells with a concomitant decrease in target protein (synaptobrevin-2/VAMP2) immunoreactivity (PubMed:14504267). Toxin uptake can be blocked by the appropriate synaptotagmin protein fragments and gangliosides in cell culture and in mice (PubMed:14504267, PubMed:15123599). BoNT/B is a 'coincidence detector'; it requires simultaneous binding to coreceptor GT1b and low pH to transform into a membrane-bound, oligomeric channel (PubMed:21925111, PubMed:22720883). Whole toxin only has protease activity after reduction which releases LC (PubMed:1331807, PubMed:7803399).