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SDS-Page

PRH1 recombinant protein

PRH1, 17-122aa, Human, His tag, E Coli

Gene Names
PRH1; PA; Db-s; PRH2; PIF-S; Pr1/Pr2
Applications
SDS-Page
Purity
> 80% by SDS-PAGE
Synonyms
PRH1; 17-122aa; Human; His tag; E Coli; Proline-rich protein HaeIII subfamily 1 precursor; PA; PRH1 recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 80% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT
Concentration
0.25 mg/ml (determined by BCA assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSQDLNEDV SQEDVPLVIS DGGDSEQFLD EERQGPPLGG QQSQPSAGDG NQDDGPQQGP PQQGGQQQQG PPPPQGKPQG PPQQGGHPPP PQGRPQGPPQ QGGHPRPPR
Sequence Length
166
Applicable Applications for PRH1 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

SDS-Page

SDS-Page
Related Product Information for PRH1 recombinant protein
PRH1 act as highly potent inhibitors of crystal growth of calcium phosphates. They provide a protective and reparative environment for dental enamel which is important for the integrity of the teeth. Recombinant human PRH1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for PRH1 recombinant protein
References
Friedman,R.D., et al. (1975) Am. J. Hum. Genet. 27 (3), 304-314

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13.4kDa (129aa), confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
NCBI Official Synonym Full Names
proline rich protein HaeIII subfamily 1
NCBI Official Symbol
PRH1
NCBI Official Synonym Symbols
PA; Db-s; PRH2; PIF-S; Pr1/Pr2
NCBI Protein Information
salivary acidic proline-rich phosphoprotein 1/2
UniProt Protein Name
Salivary acidic proline-rich phosphoprotein 1/2
UniProt Gene Name
PRH1
UniProt Synonym Gene Names
Pa; PIF-S

NCBI Description

This gene encodes a member of the heterogeneous family of proline-rich salivary glycoproteins. The encoded preproprotein undergoes proteolytic processing to generate one or more mature isoforms before secretion from the parotid and submandibular/sublingual glands. Multiple distinct alleles of this locus including the parotid isoelectric-focusing variant slow (PIF-s), the parotid acidic protein (Pa), and the double band slow (Db-s) isoforms have been characterized. The reference genome encodes the Db-s allele. Certain alleles of this gene are associated with susceptibility to dental caries. This gene is located in a cluster of closely related salivary proline-rich proteins on chromosome 12. Co-transcription of this gene with adjacent genes has been observed. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2015]

Uniprot Description

PRP's act as highly potent inhibitors of crystal growth of calcium phosphates. They provide a protective and reparative environment for dental enamel which is important for the integrity of the teeth.

Research Articles on PRH1

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Product Notes

The PRH1 prh1 (Catalog #AAA204857) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's PRH1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the PRH1 prh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSQDLNEDV SQEDVPLVIS DGGDSEQFLD EERQGPPLGG QQSQPSAGDG NQDDGPQQGP PQQGGQQQQG PPPPQGKPQG PPQQGGHPPP PQGRPQGPPQ QGGHPRPPR. It is sometimes possible for the material contained within the vial of "PRH1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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