Chromogranin-A Recombinant Protein | CHGA recombinant protein

Chromogranin-A, 19-457aa, Human, His tag, E Coli

Cat. Num. AAA203324

• Gene Names: CHGA; CGA
• Applications: SDS-Page
• Purity: > 80% by SDS-PAGE
• Synonyms: Chromogranin-A; 19-457aa; Human; His tag; E Coli; CgA; CHGA; Pituitary secretory protein I (SP1); beta Granin; CHG A; Chromogranin A precursor; SP I; Chromogranin A parathyroid secretory protein 1; ChromograninA; Pancreastatin; Parastatin; Parathyroid secretory protein 1; SP1; SPI; Vasostatin; Pituitary secretory protein I; ; CHGA recombinant protein

• Host: E Coli
• Purity/Purification: > 80% by SDS-PAGE
• Form/Format: 20mM Tris-HCl (pH .7.5) containing 2mM EDTA, 0.1mM PMSF, 10% glycerol
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MLPVNSPMNK GDTEVMKCIV EVISDTLSKP SPMPVSQECF ETLRGDERIL SILRHQNLLK ELQDLALQGA KERAHQQKKH SGFEDELSEV LENQSSQAEL KEAVEEPSSK DVMEKREDSK EAEKSGEATD GARPQALPEP MQESKAEGNN QAPGEEEEEE EEATNTHPPA SLPSQKYPGP QAEGDSEGLS QGLVDREKGL SAEPGWQAKR EEEEEEEEEA EAGEEAVPEE EGPTVVLNPH PSLGYKEIRK GESRSEALAV DGAGKPGAEE AQDPEGKGEQ EHSQQKEEEE EMAVVPQGLF RGGKSGELEQ EEERLSKEWE DSKRWSKMDQ LAKELTAEKR LEGQEEEEDN RDSSMKLSFR ARAYGFRGPG PQLRRGWRPS SREDSLEAGL PLQVRGYPEE KKEEEGSANR RPEDQELESL SAIEAELEKV AHQLQALRRG
• Sequence Length: 457

• Applicable Applications for CHGA recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for CHGA recombinant protein

Chromogranin A is a member of the chromogranin/secretogranin (granins) family of neuroendocrine secretory proteins. Chromogranin A (CgA) represents the major soluble protein co-stored and co-released with catecholamines, within chromaffin cells secretory granules. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. It is considered a prognostic marker of several diseases, including a variety of tumours and cardiac heart failure. Recombinant human Chromogranin A, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.

Product Categories/Family for CHGA recombinant protein

Cancer

References

Montero-Hadjadje M., et al. (2009). J Biol Chem. 284 (18):12420-31.; Hendy GN., et al. (1995). Clin Invest Med. 18 (1):47-65;

NCBI and Uniprot Product Information

• NCBI GI #: 4502805
• NCBI GeneID: 1113
• NCBI Accession #: NP_001266
• NCBI GenBank Nucleotide #: NM_001275.3
• UniProt Accession #: P10645; Q53FA8; Q6NR84; Q96E84; Q96GL7; Q9BQB5; B2R9E9
• Molecular Weight: 51.2 kDa (460aa) (Molecular weight on SDS-PAGE will appear higher)
• NCBI Official Full Name: chromogranin-A isoform 1 preproprotein
• NCBI Official Synonym Full Names: chromogranin A
• NCBI Official Symbol: CHGA
• NCBI Official Synonym Symbols: CGA
• NCBI Protein Information: chromogranin-A
• UniProt Protein Name: Chromogranin-A
• Protein Family: Chromogranin
• UniProt Gene Name: CHGA
• UniProt Synonym Gene Names: CgA; SP-I

NCBI Description

The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Two other peptides, catestatin and chromofungin, have antimicrobial activity and antifungal activity, respectively. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

Pancreastatin: Strongly inhibits glucose induced insulin release from the pancreas.Catestatin: Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist (PubMed:15326220). Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa (PubMed:15723172 and PubMed:24723458). Can induce mast cell migration, degranulation and production of cytokines and chemokines (PubMed:21214543). Acts as a potent scavenger of free radicals in vitro (PubMed:24723458). May play a role in the regulation of cardiac function and blood pressure (PubMed:18541522).