Chicken A Disintegrin And Metalloprotease 8 ELISA Kit | ADAM8 elisa kit

Chicken A Disintegrin And Metalloprotease 8 ELISA Kit

Cat. Num. AAA739304

• Gene Names: ADAM8; MS2; CD156; CD156a
• Reactivity: Chicken
• Synonyms: A Disintegrin And Metalloprotease 8; Chicken A Disintegrin And Metalloprotease 8 ELISA Kit; ADAM8 elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This ADAM8 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken ADAM8. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADAM8 elisa kit

Principle of the Assay: ADAM8 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADAM8 antibody and an ADAM8-HRP conjugate. The assay sample and buffer are incubated together with ADAM8-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADAM8 concentration since ADAM8 from samples and ADAM8-HRP conjugate compete for the anti-ADAM8 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADAM8 from the sample, fewer sites are left to bind ADAM8-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAM8 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ADAM8 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 109731932
• NCBI GeneID: 101
• Molecular Weight: 80,262 Da
• NCBI Official Full Name: ADAM8 protein
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 8
• NCBI Official Symbol: ADAM8
• NCBI Official Synonym Symbols: MS2; CD156; CD156a
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 8; cell surface antigen MS2; human leukocyte differentiation antigen; a disintegrin and metalloproteinase domain 8
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 8
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM8
• UniProt Synonym Gene Names: MS2; ADAM 8
• UniProt Entry Name: ADAM8_HUMAN

NCBI Description

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene may be involved in cell adhesion during neurodegeneration, and it is thought to be a target for allergic respiratory diseases, including asthma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2009]

Uniprot Description

ADAM8: Possible involvement in extravasation of leukocytes.

Protein type: Membrane protein, integral; EC 3.4.24.-; Motility/polarity/chemotaxis; Protease

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: specific granule; cell surface; integral to plasma membrane; cytoplasm; plasma membrane; podosome; dense core granule membrane

Molecular Function: protein binding; protein self-association; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity; calcium ion binding; cell adhesion molecule binding

Biological Process: extracellular matrix organization and biogenesis; positive regulation of cell adhesion; cell morphogenesis; proteolysis; positive regulation of membrane protein ectodomain proteolysis; leukocyte migration during inflammatory response; activation of NF-kappaB transcription factor; positive regulation of T cell differentiation in the thymus; extracellular matrix disassembly; positive regulation of protein kinase B signaling cascade; cell-cell adhesion; regulation of cell-cell adhesion; positive regulation of protein secretion; positive regulation of acute inflammatory response; positive regulation of bone resorption; angiogenesis; negative regulation of neuron apoptosis; inflammatory response; lymphocyte chemotaxis