Typical Testing Data/Standard Curve (for reference only)
Human Choline acetyltransferase ELISA Kit | ChAT elisa kit
Human Choline acetyltransferase ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CH3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CH3 antibody and an CH3-HRP conjugate. The assay sample and buffer are incubated together with CH3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CH3 concentration since CH3 from samples and CH3-HRP conjugate compete for the anti-CH3 antibody binding site. Since the number of sites is limited, as more sites are occupied by CH3 from the sample, fewer sites are left to bind CH3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CH3 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]
Uniprot Description
CHAT: an enzyme that catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Four alternative splice variants have been described.
Protein type: Acetyltransferase; Lipid Metabolism - glycerophospholipid; EC 2.3.1.6
Chromosomal Location of Human Ortholog: 10q11.2
Cellular Component: mitochondrion; cell soma; axon; cytoplasm; cytosol; nucleus
Molecular Function: choline O-acetyltransferase activity
Biological Process: synaptic transmission; rhythmic behavior; rhythmic excitation; muscle development; phospholipid metabolic process; neurotransmitter secretion; glycerophospholipid biosynthetic process; dendrite development; phosphatidylcholine biosynthetic process; neuromuscular synaptic transmission; establishment of synaptic specificity at neuromuscular junction; adult walking behavior; neurotransmitter biosynthetic process
Disease: Myasthenic Syndrome, Congenital, Associated With Episodic Apnea
Research Articles on ChAT
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Product Notes
The Human ChAT chat (Catalog #AAA725496) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA725496 ELISA Kit recognizes Human ChAT. It is sometimes possible for the material contained within the vial of "Choline acetyltransferase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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