Typical Testing Data/Standard Curve
Human Cytochrome c oxidase subunit 6C (COX6C) ELISA Kit | COX6C elisa kit
Human Cytochrome c oxidase subunit 6C (COX6C) ELISA Kit
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Principle of the Assay||COX6C ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-COX6C antibody and an COX6C-HRP conjugate. The assay sample and buffer are incubated together with COX6C-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COX6C concentration since COX6C from samples and COX6C-HRP conjugate compete for the anti-COX6C antibody binding site. Since the number of sites is limited, as more sites are occupied by COX6C from the sample, fewer sites are left to bind COX6C-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COX6C concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Cytochrome c oxidase, the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes subunit VIc, which has 77% amino acid sequence identity with mouse subunit VIc. This gene is up-regulated in prostate cancer cells. A pseudogene has been found on chromosomes 16p12. [provided by RefSeq, Jul 2010]
Uniprot Description
COX6C: This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. Belongs to the cytochrome c oxidase subunit 6c family.
Protein type: Energy Metabolism - oxidative phosphorylation; Mitochondrial; Oxidoreductase; Membrane protein, integral; EC 1.9.3.1
Chromosomal Location of Human Ortholog: 8q22.2
Cellular Component: mitochondrion; mitochondrial inner membrane; integral to membrane
Molecular Function: cytochrome-c oxidase activity
Biological Process: cellular metabolic process; generation of precursor metabolites and energy; transmembrane transport
Research Articles on COX6C
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Product Notes
The Human COX6C cox6c (Catalog #AAA7230628) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7230628 ELISA Kit recognizes Human COX6C. It is sometimes possible for the material contained within the vial of "Cytochrome c oxidase subunit 6C (COX6C), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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