CD19 / Leu-12 recombinant protein

Recombinant Mouse CD19 / Leu-12 Protein (His tag)

Cat. Num. AAA2546942

• Gene Names: Ces2c; Ces2; CES 2; ACH M1; ces2A3
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: CD19 / Leu-12; Recombinant Mouse CD19 / Leu-12 Protein (His tag); AW495831; CD19 / Leu-12 recombinant protein

• Host: Human Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 561

• Application Notes: The recombinant mouse CD19 consists of 282 amino acids and has a predicted molecular mass of 31 kDa.
• Predicted N Terminal: Gly 17
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for CD19 / Leu-12 recombinant protein

Background: The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 19 (CD19) is a member of CD system. CD19 is a cell surface molecule that assembles with the antigen receptor of B-cells. This results in a descent in threshold for antigen receptor-dependent stimulation. A simplified view holds that the ability of B-cells to respond to the various antigens in a specific and sensitive manner is achieved in the presence of low-affinity antigen receptors. CD19 primarily acts as a B-cell coreceptor in conjunction with CD21 and CD81. The formation of the receptor complex is induced by antigen and CD19, induced by exogenous antigen, has been found cytoplasmic tail phosphorylated and bind to sIg.

Description: A DNA sequence encoding the extracellular domain of mouse CD19 (NP_663578.1) (Met 1-Gly 287) was expressed, with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 21704206
• NCBI GeneID: 234671
• NCBI Accession #: NP_663578.1
• NCBI GenBank Nucleotide #: NM_145603.2
• UniProt Accession #: Q91WG0
• Molecular Weight: 62,470 Da
• NCBI Official Full Name: acylcarnitine hydrolase
• NCBI Official Synonym Full Names: carboxylesterase 2C
• NCBI Official Symbol: Ces2c
• NCBI Official Synonym Symbols: Ces2; CES 2; ACH M1; ces2A3
• NCBI Protein Information: acylcarnitine hydrolase
• UniProt Protein Name: Acylcarnitine hydrolase
• UniProt Gene Name: Ces2c
• UniProt Synonym Gene Names: Ces2; ACH M1; CES 2

NCBI Description

This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. The protein encoded by this gene is the major intestinal enzyme and functions in intestine drug clearance. The transcription of this gene is regulated by several factors including HNF-4alpha (hepatocyte nuclear factor-4alpha), Sp1 (specificity protein 1), Sp3 and USF1 (upstream stimulatory factor 1). The expression and activity of this gene is age-related but independent of growth hormone level. This gene is clustered with several family members including a few of pseudogenes and Ces5 on chromosome 8.[provided by RefSeq, Jun 2010]

Uniprot Description

CES2: Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Shows high catalytic efficiency for hydrolysis of cocaine, 4-methylumbelliferyl acetate, heroin and 6-monoacetylmorphine. Belongs to the type-B carboxylesterase/lipase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.1.1; EC 3.1.1.56; EC 3.1.1.84; Endoplasmic reticulum; Hydrolase; Xenobiotic Metabolism - drug metabolism - other enzymes

Chromosomal Location of Human Ortholog: 8|8 D3

Cellular Component: extracellular space; intracellular membrane-bound organelle

Molecular Function: acylcarnitine hydrolase activity

Biological Process: response to acid