carboxylesterase 2/CES2 Active Protein | CES2 active protein

carboxylesterase 2/CES2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120236

• Gene Names: CES2; iCE; CE-2; PCE-2; CES2A1
• Purity: >95% as determined by SDS-PAGE
• Synonyms: carboxylesterase 2/CES2; carboxylesterase 2/CES2 Protein; Human; Recombinant (His Tag); Human CES2/Carboxylesterase-2 Protein (His Tag); CE-2 Protein; CES2A1 Protein; iCE Protein; PCE-2 Protein; carboxylesterase 2; CES2 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM NaAc, 150mM NaCl, 10% Glycerol, pH5.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Leu559

• Species: Human
• Activity: Measured by its ability to hydrolyze p-nitrophenylacetate. The specific activity is >20000 pmols/min/ug.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 27
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human CES2 isoform 1 (O00748-1)(Met 1-Leu 559) was expressed, with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CES2 active protein

Background: Carboxylesterase 2 (CES2) is a member of the carboxylesterase family and belongs to the multigene family. Carboxylesterase 2 is responsible for the hydrolysis of ester- and amide-bond-containing drugs such as cocaine and beroin. It also serves to hydrolyze long-chain fatty acid esters and thioesters. It is speculated that carboxylesterases may play a role in lipid metabolism and the blood-brain barrier system and together with isform 1, are a serine esterase involved in both drug metabolism and activation. Human carboxylesterase 2 is commonly expressed in tumor tissues and irinotecan, a topoisomerase I inhibitor commonly used in the treatment of many solid tumors.

References

Imai T. et al. (2006) Human carboxylesterase isozymes: catalytic properties and rational drug design. Drug metab pharmacokinet. 21 (3): 173-85.Guang Xu, et al. (2002) Human carboxylesterase 2 is commonly expressed in tumor tissue and is correlated with activation of irinotecan. Clin Cancer Res. 8: 2605.Zhang, et al. (2002) Comprehensive Evaluation of Carboxylesterase-2 Expression in Normal Human Tissues Using Tissue Array Analysis. Applied Immunohistochemistry & Molecular Morphology. 10 (4): 374-80.

NCBI and Uniprot Product Information

• NCBI GI #: 37622885
• NCBI GeneID: 8824
• NCBI Accession #: NP_003860.2
• NCBI GenBank Nucleotide #: NM_003869.5
• UniProt Accession #: O00748; Q16859; Q5MAB8; Q7Z366; Q8IUP4; Q8TCP8
• Molecular Weight: 61,807 Da
• NCBI Official Full Name: cocaine esterase isoform 1
• NCBI Official Synonym Full Names: carboxylesterase 2
• NCBI Official Symbol: CES2
• NCBI Official Synonym Symbols: iCE; CE-2; PCE-2; CES2A1
• NCBI Protein Information: cocaine esterase; hCE-2; carboxylesterase 2 (intestine, liver); methylumbelliferyl-acetate deacetylase 2; intestinal carboxylesterase; liver carboxylesterase-2
• UniProt Protein Name: Cocaine esterase
• Protein Family: Liver carboxylesterase
• UniProt Gene Name: CES2
• UniProt Synonym Gene Names: ICE; CE-2; hCE-2
• UniProt Entry Name: EST2_HUMAN

NCBI Description

This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. The protein encoded by this gene is the major intestinal enzyme and functions in intestine drug clearance. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Oct 2010]

Uniprot Description

CES2: Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Shows high catalytic efficiency for hydrolysis of cocaine, 4-methylumbelliferyl acetate, heroin and 6-monoacetylmorphine. Belongs to the type-B carboxylesterase/lipase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.1.56; EC 3.1.1.84; Xenobiotic Metabolism - drug metabolism - other enzymes; Endoplasmic reticulum; EC 3.1.1.1; Hydrolase

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: extracellular space; endoplasmic reticulum; endoplasmic reticulum lumen

Molecular Function: methylumbelliferyl-acetate deacetylase activity

Biological Process: catabolic process