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SDS-Page

Cadherin EGF LAG Seven Pass G-Type Receptor 3 Recombinant Protein | CELSR3 recombinant protein

Recombinant Cadherin EGF LAG Seven Pass G-Type Receptor 3 (CELSR3)

Gene Names
CELSR3; FMI1; EGFL1; HFMI1; MEGF2; ADGRC3; CDHF11; RESDA1
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
>95%
Synonyms
Cadherin EGF LAG Seven Pass G-Type Receptor 3; Recombinant Cadherin EGF LAG Seven Pass G-Type Receptor 3 (CELSR3); CELSR3 recombinant protein
Ordering
For Research Use Only!
Host
Host: E Coli
Source: Prokaryotic expression
Purity/Purification
>95%
Form/Format
Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
SNRSDTFPSG IIGRIPAYDP DVSDHLFYSF ERGNELQLLV VNQTSGELRL SRKLDNNRPL VASMLVTVTD GLHSVTAQCV LRVVIITEEL LANSLTVRLE NMWQERFLSP LLGRFLEGVA AVLATPAEDV FIFNIQNDTD VGGTVLNVSF SALAPRGAGA GAAGPWFSSE ELQEQLYVRR AALAARSLLD VLPFDDNVCL REPCENYMKC VSVLRFDSSA PFLASASTLF RPIQPIAGLR C
Sequence Length
3312
Applicable Applications for CELSR3 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Predicted Molecular Mass
31.3kDa
Accurate Molecular Mass (KD)
31kDa
Endotoxin
<1.0EU per 1ug (determined by the LAL method)
Expression System
Prokaryotic expression
Tag
N-terminal His-Tag, T7 tag
Organism Species
Homo sapiens (Human)
Fragment
Ser2~Phe137
Usage
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
358,640 Da
NCBI Official Full Name
cadherin EGF LAG seven-pass G-type receptor 3
NCBI Official Synonym Full Names
cadherin, EGF LAG seven-pass G-type receptor 3
NCBI Official Symbol
CELSR3
NCBI Official Synonym Symbols
FMI1; EGFL1; HFMI1; MEGF2; ADGRC3; CDHF11; RESDA1
NCBI Protein Information
cadherin EGF LAG seven-pass G-type receptor 3
UniProt Protein Name
Cadherin EGF LAG seven-pass G-type receptor 3
UniProt Gene Name
CELSR3
UniProt Synonym Gene Names
CDHF11; EGFL1; FMI1; KIAA0812; MEGF2; EGF-like protein 1; hFmi1; Multiple EGF-like domains protein 2
UniProt Entry Name
CELR3_HUMAN

NCBI Description

This gene belongs to the flamingo subfamily, which is included in the cadherin superfamily. The flamingo cadherins consist of nonclassic-type cadherins that do not interact with catenins. They are plasma membrane proteins containing seven epidermal growth factor-like repeats, nine cadherin domains and two laminin A G-type repeats in their ectodomain. They also have seven transmembrane domains, a characteristic feature of their subfamily. The encoded protein may be involved in the regulation of contact-dependent neurite growth and may play a role in tumor formation. [provided by RefSeq, Jun 2013]

Uniprot Description

CELSR3: Receptor that may have an important role in cell/cell signaling during nervous system formation. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 2

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: plasma membrane; integral to membrane

Molecular Function: G-protein coupled receptor activity; protein binding; calcium ion binding

Biological Process: G-protein coupled receptor protein signaling pathway; regulation of protein localization; axonal fasciculation; neuron migration; cilium biogenesis; homophilic cell adhesion

Research Articles on CELSR3

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Product Notes

The CELSR3 celsr3 (Catalog #AAA2031261) is a Recombinant Protein produced from Host: E Coli Source: Prokaryotic expression and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Cadherin EGF LAG Seven Pass G-Type Receptor 3 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the CELSR3 celsr3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
SNRSDTFPS G IIGRIPAYDP DVSDHLFYSF ERGNELQLLV VNQTSGELRL SRKLDNNRPL VASMLVTVTD GLHSVTAQCV LRVVIITEEL LANSLTVRLE NMWQERFLSP LLGRFLEGVA AVLATPAEDV FIFNIQNDTD VGGTVLNVSF SALAPRGAGA GAAGPWFSSE ELQEQLYVRR AALAARSLLD VLPFDDNVCL REPCENYMKC VSVLRFDSSA PFLASASTLF RPIQPIAGLR C
. It is sometimes possible for the material contained within the vial of "Cadherin EGF LAG Seven Pass G-Type Receptor 3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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