Testing Data
T47D/GFP Cell Line
T47D/GFP Cell Line
Synonyms
T47D/GFP; T47D/GFP Cell Line; T47D/GFP cell line
Concentration
1 mL, 1 x 10^6 cells/mL in 70% DMEM, 20% FBS, 10% DMSO (varies by lot)
Methods
Establishing T47D/GFP Cultures from Frozen Cells
1. Place 10 mL of complete DMEM growth medium in a 50-mL conical tube. Thaw the frozen cryovial of cells within 1-2 minutes by gentle agitation in a 37 degree C water bath. Decontaminate the cryovial by wiping the surface of the vial with 70% (v/v) ethanol.
2. Transfer the thawed cell suspension to the conical tube containing 10 ml of growth medium.
3. Collect the cells by centrifugation at 1000 rpm for 5 minutes at room temperature. Remove the growth medium by aspiration.
4. Resuspend the cells in the conical tube in 15 mL of fresh growth medium by gently pipetting up and down.
5. Transfer the 15 mL of cell suspension to a T-75 tissue culture flask. Place the cells in a 37 degree C incubator at 5% CO2.
6. Monitor cell density daily. Cells should be passaged when the culture reaches 95% confluence.
1. Place 10 mL of complete DMEM growth medium in a 50-mL conical tube. Thaw the frozen cryovial of cells within 1-2 minutes by gentle agitation in a 37 degree C water bath. Decontaminate the cryovial by wiping the surface of the vial with 70% (v/v) ethanol.
2. Transfer the thawed cell suspension to the conical tube containing 10 ml of growth medium.
3. Collect the cells by centrifugation at 1000 rpm for 5 minutes at room temperature. Remove the growth medium by aspiration.
4. Resuspend the cells in the conical tube in 15 mL of fresh growth medium by gently pipetting up and down.
5. Transfer the 15 mL of cell suspension to a T-75 tissue culture flask. Place the cells in a 37 degree C incubator at 5% CO2.
6. Monitor cell density daily. Cells should be passaged when the culture reaches 95% confluence.
Medium
1. Culture Medium: D-MEM (high glucose), 10% fetal bovine serum (FBS), 0.1 mM MEM Non-Essential Amino Acids (NEAA), 2 mM L-glutamine, 1% Pen-Strep.
2. Freeze Medium: 70% DMEM, 20% FBS, 10% DMSO.
2. Freeze Medium: 70% DMEM, 20% FBS, 10% DMSO.
Shipping Note
Product available only in the USA.
Quality Control
This cryovial contains at least 1.0 × 10^6 T47D/GFP cells as determined by morphology, trypan-blue dye exclusion, and viable cell count. The T47D/GFP cells are tested free of microbial contamination.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Liquid nitrogen
Note: For best results begin culture of cells immediately upon receipt. If this is not possible, store at -80 degree C until first culture. Store subsequent cultured cells long term in liquid nitrogen.
Note: For best results begin culture of cells immediately upon receipt. If this is not possible, store at -80 degree C until first culture. Store subsequent cultured cells long term in liquid nitrogen.
Testing Data
Testing Data
Related Product Information for T47D/GFP cell line
• These cell lines stably express one or more reporter molecules.
The T-47 line was isolated in 1979 from a pleural effusion obtained from a 54 year old female patient with an infiltrating ductal carcinoma of the breast. This differentiated epithelial cell line was found to contain cytoplasmic junctions and receptors to 17 beta estradiol, other steroids and calcitonin. These cells exhibit epithelial morphology and form monolayers in culture. The cell line has abundant activity in both the Boyden chamber chemoinvasion and chemotaxis assay. The T47D cell line is also able to grow on agarose, an indicator of transformation and tumorigenicity, and displays a relatively high colony forming efficiency.
Our T47D/GFP cell line stably expresses GFP; the gene was introduced using lentivirus.
The T-47 line was isolated in 1979 from a pleural effusion obtained from a 54 year old female patient with an infiltrating ductal carcinoma of the breast. This differentiated epithelial cell line was found to contain cytoplasmic junctions and receptors to 17 beta estradiol, other steroids and calcitonin. These cells exhibit epithelial morphology and form monolayers in culture. The cell line has abundant activity in both the Boyden chamber chemoinvasion and chemotaxis assay. The T47D cell line is also able to grow on agarose, an indicator of transformation and tumorigenicity, and displays a relatively high colony forming efficiency.
Our T47D/GFP cell line stably expresses GFP; the gene was introduced using lentivirus.
Product Categories/Family for T47D/GFP cell line
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Product Notes
The T47D/GFP (Catalog #AAA168156) is a Cell Line and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "T47D/GFP, Cell Line" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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