Typical Testing Data/Standard Curve (for reference only)
(Human CEA PicoKine ELISA Kit standard curve )
Human CEA ELISA Kit | CEACAM5 elisa kit
Human CEA PicoKine ELISA Kit
Gene Names
CEACAM5; CEA; CD66e
Reactivity
Human
Synonyms
CEA; Human CEA PicoKine ELISA Kit; Carcinoembryonic antigen-related cell adhesion molecule 5; carcinoembryonic antigen-related cell adhesion molecule 5; Carcinoembryonic antigen ; Carcinoembryonic antigen related cell adhesion molecule 5; CD66e; CD66e antigen; CEACAM 5; CEACAM5; DKFZp781M2392; Meconium antigen 100; CEACAM5 elisa kit
Reactivity
Human
Specificity
Natural and recombinant human CEA
Sequence Length
702
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
312pg/ml-20,000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Human CEA PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Human CEA PicoKine ELISA Kit standard curve )
Related Product Information for CEACAM5 elisa kit
Principle of the assay: human CEA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CEA has been precoated onto 96-well plates. Standards(NSO, K35-A685) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CEA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CEA amount of sample captured in plate.
Background: Carcinoembryonic antigen(CEA) is a complex immunoreactive glycoprotein with a molecular weight of 180,000 comprising 60% carbohydrate. It is found in adenocarcinomas of endodermally derived digestive system epithelia and in fetal colon.Carcinoembryonic antigen is one of the most widely used tumor markers in serum immunoassay determinations of carcinoma.
Background: Carcinoembryonic antigen(CEA) is a complex immunoreactive glycoprotein with a molecular weight of 180,000 comprising 60% carbohydrate. It is found in adenocarcinomas of endodermally derived digestive system epithelia and in fetal colon.Carcinoembryonic antigen is one of the most widely used tumor markers in serum immunoassay determinations of carcinoma.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
76,724 Da
NCBI Official Full Name
Carcinoembryonic antigen-related cell adhesion molecule 5
NCBI Official Synonym Full Names
carcinoembryonic antigen-related cell adhesion molecule 5
NCBI Official Symbol
CEACAM5
NCBI Official Synonym Symbols
CEA; CD66e
NCBI Protein Information
carcinoembryonic antigen-related cell adhesion molecule 5; meconium antigen 100
UniProt Protein Name
Carcinoembryonic antigen-related cell adhesion molecule 5
Protein Family
UniProt Gene Name
CEACAM5
UniProt Synonym Gene Names
CEA; CEA
UniProt Entry Name
CEAM5_HUMAN
NCBI Description
Members of the CEACAM subfamily, including CEACAM5, belong to the CEA gene family. For general information on the CEA gene family, see CEACAM1 (MIM 109770).[supplied by OMIM, Oct 2009]
Uniprot Description
CEACAM5: Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins. Homodimer. Binding of E.coli Dr adhesins leads to dissociation of the homodimer. Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon. Belongs to the immunoglobulin superfamily. CEA family.
Protein type: Membrane protein, GPI anchor; Immunoglobulin superfamily; Membrane protein, integral
Chromosomal Location of Human Ortholog: 19q13.1-q13.2
Cellular Component: integral to plasma membrane; basolateral plasma membrane
Molecular Function: identical protein binding; protein homodimerization activity
Biological Process: homotypic cell-cell adhesion; negative regulation of apoptosis
Research Articles on CEACAM5
Similar Products
Product Notes
The Human CEACAM5 ceacam5 (Catalog #AAA175772) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA175772 ELISA Kit recognizes Human CEACAM5. It is sometimes possible for the material contained within the vial of "CEA, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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