Universal Tissue cDNA, Reverse Transcribed by Random Hexamer Adult Normal, BioGenomics cDNA Clone
Universal Tissue cDNA, Reverse Transcribed by Random Hexamer, Mouse Adult Normal, BioGenomics
Purity
Molecular Biology Grade
Synonyms
Universal Tissue cDNA; Reverse Transcribed by Random Hexamer Adult Normal; BioGenomics; Reverse Transcribed by Random Hexamer; Mouse Adult Normal; BioGenomics cdna clone
Purity/Purification
Molecular Biology Grade
Form/Format
Supplied as a liquid in 1X RT buffer: 50mM Tris-Cl, pH 8.3, 75mM KCl, 3mM MgCl2, 10mM DTT.
Concentration
2.5ng/ul; 1ul cDNA is sufficient for one PCR reaction. (varies by lot)
Application Notes
Suitable for use in SNP analysis, Southern Blot, PCR, Genomic DNA library construction, Profiling study in gene expression.
Supplied With
Tissue, cDNA, Actin Control Primer
BioGenomics™ Universal cDNA
Mixed Male and Female donors. Prepared by reverse transcribed RNA by using random hexamer or oligo dT primer. Total RNA is isolated by modified guanidine thiocyanate techniques. The Universal cDNA serves as a standard for comparison of gene expression by real time PCR and regular PCR, and also as a gene pool for cloning genes.
Species Selection
Mouse Universal cDNA is prepared from several male and female Balb/C mice whole bodies without fur.
Donor Information
Pool of 5 normal male mice donors, 5-6 weeks old.
Pool of 16 normal female mice donors, 5-6 weeks old.
Pool of 16 normal female mice donors, 5-6 weeks old.
Quality Control
RNA Integrity for cDNA synthesis examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA using denaturing agarose gel. Quality and purity of total RNA is determined spectrophotometrically.
A260/280 1.8 - 2.0 (10mM Tris-Cl, pH 7.5) 8S/18S Ratio > 1
• RNA used for cDNA synthesis is treated by DNase I and is tested as DNA-free RNA by PCR.
• The synthesized human, animal, and cell line cDNA was 5' selected to ensure its full length.
• The cDNA was used as template for PCR amplification of beta-actin gene and an 838bp beta-actin band was visualized on 1% or 2% agarose gel. Control primer is included and is enough for 10 PCR Reactions.
A260/280 1.8 - 2.0 (10mM Tris-Cl, pH 7.5) 8S/18S Ratio > 1
• RNA used for cDNA synthesis is treated by DNase I and is tested as DNA-free RNA by PCR.
• The synthesized human, animal, and cell line cDNA was 5' selected to ensure its full length.
• The cDNA was used as template for PCR amplification of beta-actin gene and an 838bp beta-actin band was visualized on 1% or 2% agarose gel. Control primer is included and is enough for 10 PCR Reactions.
Control PCR Component
Taq Polymerase: 0.2ul
10X PCR buffer: 2.5ul
10mM dNTP: 0.5ul
Control primers (5uM): 1.0ul
PCR Ready First Strand cDNA: 1.0ul
Sterile ddH2O: 19.8ul
Total Volume: 25.0 ul
10X PCR buffer: 2.5ul
10mM dNTP: 0.5ul
Control primers (5uM): 1.0ul
PCR Ready First Strand cDNA: 1.0ul
Sterile ddH2O: 19.8ul
Total Volume: 25.0 ul
Control PCR Conditions
1. 94°C x 2 minutes, 1 cycle.
2. 94°C x 30 seconds, 55°C x 30 seconds, 72°C x 30 seconds, 35 cycles.
3. 72°C x 5 minutes, 1 cycle. Hold at 4°C.
2. 94°C x 30 seconds, 55°C x 30 seconds, 72°C x 30 seconds, 35 cycles.
3. 72°C x 5 minutes, 1 cycle. Hold at 4°C.
Preparation and Storage
Aliquot to avoid repeated freezing and thawing. Store at -20°C.
Aliquots are stable for 6 months after receipt at -20°C.
For maximum recovery, centrifuge the original vial after thawing and prior to removing the cap.
Aliquots are stable for 6 months after receipt at -20°C.
For maximum recovery, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for Universal Tissue cDNA, Reverse Transcribed by Random Hexamer Adult Normal, BioGenomics cdna clone
cDNA is supplied as First Strand, Multiple Tissue Panels, and Matched Pairs. PCR-ready First Strand cDNA is tissue specific and are ready-to-use for gene discovery or expression analysis. Over 350 cDNAs from human adult and fetal normal tissues, human diseased and tumor tissues, rat, mouse, monkey and plant tissues are included in this extensive collection.
Product Categories/Family for Universal Tissue cDNA, Reverse Transcribed by Random Hexamer Adult Normal, BioGenomics cdna clone
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Product Notes
The Universal Tissue cDNA, Reverse Transcribed by Random Hexamer Adult Normal, BioGenomics (Catalog #AAA652333) is a cDNA Clone and is intended for research purposes only. The product is available for immediate purchase. Suitable for use in SNP analysis, Southern Blot, PCR, Genomic DNA library construction, Profiling study in gene expression. Researchers should empirically determine the suitability of the Universal Tissue cDNA, Reverse Transcribed by Random Hexamer Adult Normal, BioGenomics for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Universal Tissue cDNA, Reverse Transcribed by Random Hexamer Adult Normal, BioGenomics, cDNA Clone" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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