Tissue cDNA Panel cDNA Clone
Tissue cDNA Panel (Matched Pairs), Human Primary and Matched Metastasis Tumor, Breast, BioGenomics
Purity
Molecular Biology Grade
Synonyms
Tissue cDNA Panel; Tissue cDNA Panel (Matched Pairs); Human Primary and Matched Metastasis Tumor; Breast; BioGenomics; Tissue cDNA Panel cdna clone
Purity/Purification
Molecular Biology Grade
Form/Format
cDNA in 1X RT buffer (50 mM Tris Cl, pH 8.3, 75mM KCl, 3mM MgCl2, 10mM DTT).
Application Notes
Immediate PCR amplification of known genes for their expression, Quantification of low copy gene expression in multiple tissues, Identification of tissue-specific expression of target genes, Analysis of gene expression in rare tissues, Verification of genetic mutation, Comparison of a specific gene between different tissues, Analysis of mRNA alternative splicing
Bioselect Tissue cDNA
cDNA is supplied as First Strand, Multiple Tissue Panels, and Matched Pairs. PCR-ready First Strand cDNA is tissue specific and are ready-to-use for gene discovery or expression analysis. Over 350 cDNAs from human adult and fetal normal tissues, human diseased and tumor tissues, rat, mouse, monkey and plant tissues are included in this extensive collection.
Contents
Primary Tumor 1x10rxn
Normal 1x10rxn
~11ug total RNA was primed by an oligo dT primer and reverse transcribed by MMLV reverse transcriptase in 40ul final volume. RT reaction was stopped by heating at 65 degree C for 10 minutes. 1ul cDNA is sufficient for one PCR reaction. The 5' end of human clathrin cDNA (a 6kb gene) has been amplified by PCR from all of the cDNAs.
Normal 1x10rxn
~11ug total RNA was primed by an oligo dT primer and reverse transcribed by MMLV reverse transcriptase in 40ul final volume. RT reaction was stopped by heating at 65 degree C for 10 minutes. 1ul cDNA is sufficient for one PCR reaction. The 5' end of human clathrin cDNA (a 6kb gene) has been amplified by PCR from all of the cDNAs.
Features
Ready to use for PCR quantification of gene expression
Oligo dT primer used to ensure the entire 3' end of cDNA is present
With some cDNA used as templates, 12kb PCR amplicon was obtained to ensure intact cDNAs
The largest selection of cDNAs from different tissues on the market
Placenta cDNA is included in every panel as an interpanel control
Documentation of tissues' clinical histories available (additional cost)
Economical way to get cDNA from multiple tissues
Oligo dT primer used to ensure the entire 3' end of cDNA is present
With some cDNA used as templates, 12kb PCR amplicon was obtained to ensure intact cDNAs
The largest selection of cDNAs from different tissues on the market
Placenta cDNA is included in every panel as an interpanel control
Documentation of tissues' clinical histories available (additional cost)
Economical way to get cDNA from multiple tissues
Quality Control
1. The integrity of the RNA used for cDNA synthesis is examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA when electrophoresed on a denaturing agarose gel. The quality and purity of total RNA were tested by spectrophotometer. A260/280 is between 1.8 and 2.0 (detetcted in 10mM Tris-Cl, pH 7.5). The ratio of 28S/18S is > 1.
2. The RNA used for cDNA synthesis is treated by DNase I and is tested as DNA free RNA by PCR.
3. The synthesized cDNA was 5" selected to ensure its full length. The cDNA was used as a template for PCR amplification of B-actin gene and an 838 basepair B-actin band was visualized on 2% agarose gel. B-actin control primer is included. It is enough for 10 PCR reactions.
2. The RNA used for cDNA synthesis is treated by DNase I and is tested as DNA free RNA by PCR.
3. The synthesized cDNA was 5" selected to ensure its full length. The cDNA was used as a template for PCR amplification of B-actin gene and an 838 basepair B-actin band was visualized on 2% agarose gel. B-actin control primer is included. It is enough for 10 PCR reactions.
Preparation and Storage
Store cDNA panel at -20 degree C Stable for 12 months.
Product Categories/Family for Tissue cDNA Panel cdna clone
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Product Notes
The Tissue cDNA Panel (Catalog #AAA652066) is a cDNA Clone and is intended for research purposes only. The product is available for immediate purchase. Immediate PCR amplification of known genes for their expression, Quantification of low copy gene expression in multiple tissues, Identification of tissue-specific expression of target genes, Analysis of gene expression in rare tissues, Verification of genetic mutation, Comparison of a specific gene between different tissues, Analysis of mRNA alternative splicing. Researchers should empirically determine the suitability of the Tissue cDNA Panel for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tissue cDNA Panel, cDNA Clone" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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