SMUG1 cdna clone

SMUG1

Cat. Num. AAA200873

• Gene Names: SMUG1; FDG; UNG3; HMUDG
• Synonyms: SMUG1; FDG; HMUDG; UNG3; SMUG1 cdna clone

• Form/Format: Lyophilized
• Sequence: Nucleotide Sequence: ATGCCCCAGGCTTTCCTGCTGGGGTCCATCCATGAGCCTGCAGGTGCCCTCATGGAGCCCCAGCCCTGCCCTGGAAGCTTGGCTGAGAGCTTCCTGGAGGAGGAGCTTCGGCTCAATGCTGAGCTGAGCCAGCTGCAGTTTTCGGAGCCTGTGGGCATCATCTACAATCCCGTGGAGTATGCATGGGAGCCACATCGCAACTACGTGACTCGCTACTGCCAGGGCCCCAAGGAAGTACTCTTCCTGGGCATGAACCCTGGACCTTTTGGCATGGCCCAGACTGGGGTGCCCTTTGGGGAAGTAAGCATGGTCCGGGACTGGTTGGGCATTGTGGGGCCTGTGCTGACCCCTCCCCAAGAGCATCCTAAACGACCAGTGCTGGGACTGGAGTGCCCACAGTCAGAAGTGAGTGGTGCCCGATTCTGGGGCTTTTTCCGGAACCTCTGTGGACAGCCTGAGGTCTTCTTCCATCACTGTTTTGTCCACAATCTATGCCCTCTGCTTTTCCTGGCTCCCAGCGGGCGCAACCTTACTCCTGCTGAGCTGCCTGCCAAGCAGCGAGAACAGCTTCTTGGGATCTGTGATGCAGCCCTCTGCCGGCAGGTGCAGCTGCTGGGGGTGCGGCTGGTGGTGGGAGTTGGGCGACTGGCAGAGCAGCGGGCACGACGGGCTCTGGCAGGCCTGATGCCAGAGGTCCAGGTGGAAGGGCTCCTGCATCCCTCTCCCCGTAACCCACAGGCCAACAAGGGCTGGGAGGCAGTGGCCAAGGAAAGATTGAATGAGCTGGGGCTGCTGCCACTGCTGTTGAAATGA; ; Translation Sequence: MPQAFLLGSI HEPAGALMEP QPCPGSLAES FLEEELRLNA ELSQLQFSEP VGIIYNPVEY AWEPHRNYVT RYCQGPKEVL FLGMNPGPFG MAQTGVPFGE VSMVRDWLGI VGPVLTPPQE HPKRPVLGLE CPQSEVSGAR FWGFFRNLCG QPEVFFHHCF VHNLCPLLFL APSGRNLTPA ELPAKQREQL LGICDAALCR QVQLLGVRLV VGVGRLAEQR ARRALAGLMP EVQVEGLLHP SPRNPQANKG WEAVAKERLN ELGLLPLLLK
• Sequence Length: 270

• Species: Human
• Chromosome Location: 12q13.13
• OMIM Reference Number: 607753
• cDNA Size: 813bp
• Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.
• Vector: (puc19-derived cloning vector)
• Preparation Before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid.
• Preparation and Storage: Store the plasmid at -20 degree C.

Related Product Information for SMUG1 cdna clone

SMUG1 is a protein that participates in base excision repair by removing uracil from single- and double-stranded DNA. Many alternatively spliced transcript variants exist for this gene; the full-length nature is known for some but not all of the variants.

Product Categories/Family for SMUG1 cdna clone

Epigenomics (Transcription & Translation)

NCBI and Uniprot Product Information

• NCBI GI #: 7657597
• NCBI GeneID: 23583
• NCBI Accession #: NP_055126.1
• NCBI GenBank Nucleotide #: NM_014311.2
• UniProt Accession #: Q53HV7
• NCBI Official Full Name: single-strand selective monofunctional uracil DNA glycosylase isoform 1
• NCBI Official Synonym Full Names: single-strand-selective monofunctional uracil-DNA glycosylase 1
• NCBI Official Symbol: SMUG1
• NCBI Official Synonym Symbols: FDG; UNG3; HMUDG
• NCBI Protein Information: single-strand selective monofunctional uracil DNA glycosylase
• UniProt Protein Name: Single-strand selective monofunctional uracil DNA glycosylase
• Protein Family: Single-strand selective monofunctional uracil DNA glycosylase
• UniProt Gene Name: SMUG1
• UniProt Entry Name: SMUG1_HUMAN

NCBI Description

This gene encodes a protein that participates in base excision repair by removing uracil from single- and double-stranded DNA. Many alternatively spliced transcript variants exist for this gene; the full-length nature is known for some but not all of the variants. [provided by RefSeq, Aug 2011]

Uniprot Description

SMUG1: Responsible for recognizing base lesions in the genome and initiating base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA and has a preference for single-stranded DNA substrates. The activity is greater against mismatches (U/G) than against matches (U/A). Excised uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5- hydroxymethyluracil (hmU)] in ssDNA and dsDNA but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine) and other oxidized damage. The activity is damage specificity and salt concentration-dependent. The general order of the preference for ssDNA and dsDNA is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at the low salt concentration. At the high concentration dsDNA (G pair) > dsDNA (A pair) > ssDNA. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage; Hydrolase; EC 3.2.2.-

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; nucleolus

Molecular Function: single-strand selective uracil DNA N-glycosylase activity; protein binding; uracil DNA N-glycosylase activity; DNA binding; oxidized pyrimidine base lesion DNA N-glycosylase activity; DNA N-glycosylase activity

Biological Process: base-excision repair, AP site formation; base-excision repair; depyrimidination; DNA repair; DNA catabolic process, endonucleolytic