RAP2B cdna clone

RAP2B

Cat. Num. AAA200900

• Synonyms: RAP2B; RAP2B cdna clone

• Form/Format: Lyophilized
• Sequence: Nucleotide Sequence: ATGAGAGAGTACAAAGTGGTGGTGCTGGGCTCGGGCGGCGTGGGCAAGTCCGCGCTCACCGTGCAGTTCGTGACGGGCTCCTTCATCGAGAAGTACGACCCGACCATCGAAGACTTTTACCGCAAGGAGATTGAGGTGGACTCGTCGCCGTCGGTGCTGGAGATCCTGGATACGGCGGGCACCGAGCAGTTCGCGTCCATGCGGGACCTGTACATCAAGAACGGCCAGGGCTTCATCCTGGTCTACAGCCTCGTCAACCAGCAGAGCTTCCAGGACATCAAGCCCATGCGGGACCAGATCATCCGCGTGAAGCGGTACGAGCGCGTGCCCATGATCCTGGTGGGCAACAAGGTGGACCTGGAGGGTGAGCGCGAGGTCTCGTACGGGGAGGGCAAGGCCCTGGCTGAGGAGTGGAGCTGCCCCTTCATGGAGACGTCGGCCAAAAACAAAGCCTCGGTAGACGAGCTATTTGCCGAGATCGTGCGGCAGATGAACTACGCGGCGCAGCCCAACGGCGATGAGGGCTGCTGCTCGGCCTGCGTGATCCTCTGA; ; Translation Sequence: MREYKVVVLG SGGVGKSALT VQFVTGSFIE KYDPTIEDFY RKEIEVDSSP SVLEILDTAGTEQFASMRDL YIKNGQGFIL VYSLVNQQSF QDIKPMRDQI IRVKRYERVP MILVGNKVDLEGEREVSYGE GKALAEEWSC PFMETSAKNK ASVDELFAEI VRQMNYAAQP NGDEGCCSACVIL
• Sequence Length: 183

• Species: Human
• Chromosome Location: 3q25.2
• OMIM Reference Number: 179541
• cDNA Size: 552bp
• Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.
• Vector: (puc19-derived cloning vector)
• Preparation Before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid.
• Preparation and Storage: Store the plasmid at -20 degree C.

Related Product Information for RAP2B cdna clone

RAP2B belongs to the Ras-related protein family. RAP2B is small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. It is involved in EGFR and CHRM3 signaling pathways through stimulation of PLCE1.

Product Categories/Family for RAP2B cdna clone

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 38201690
• NCBI GeneID: 5912
• NCBI Accession #: NP_002877.2
• NCBI GenBank Nucleotide #: NM_002886.3
• UniProt Accession #: P61225
• NCBI Official Full Name: ras-related protein Rap-2b
• NCBI Official Synonym Full Names: RAP2B, member of RAS oncogene family
• NCBI Official Symbol: RAP2B
• NCBI Protein Information: ras-related protein Rap-2b
• UniProt Protein Name: Ras-related protein Rap-2b
• Protein Family: Ras-related protein
• UniProt Gene Name: RAP2B
• UniProt Entry Name: RAP2B_HUMAN

NCBI Description

This intronless gene belongs to a family of RAS-related genes. The proteins encoded by these genes share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between the RAP and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. Evidence suggests that this protein may be polyisoprenylated and palmitoylated. [provided by RefSeq, Jul 2008]

Uniprot Description

RAP2B: Small GTP-binding protein which cycles between a GDP- bound inactive and a GTP-bound active form. Involved in EGFR and CHRM3 signaling pathways through stimulation of PLCE1. May play a role in cytoskeletal rearrangements and regulate cell spreading through activation of the effector TNIK. May regulate membrane vesiculation in red blood cells. Belongs to the small GTPase superfamily. Ras family.

Protein type: G protein, monomeric, Ras; G protein, monomeric; G protein

Chromosomal Location of Human Ortholog: 3q25.2

Cellular Component: tight junction; membrane; recycling endosome membrane; plasma membrane; cytosol; lipid raft

Molecular Function: protein domain specific binding; GDP binding; GTP binding

Biological Process: microvillus biogenesis; platelet activation; signal transduction; positive regulation of protein amino acid autophosphorylation; negative regulation of cell migration; Rap protein signal transduction