POLR3H cdna clone

POLR3H

Cat. Num. AAA200816

• Gene Names: POLR3H; RPC8; RPC22.9
• Synonyms: POLR3H; RPC22.9; RPC8; POLR3H cdna clone

• Form/Format: Lyophilized
• Sequence: Nucleotide Sequence: ATGTTCGTCCTGGTGGAAATGGTGGACACCGTCCGGATCCCCCCTTGGCAGTTTGAGAGGAAGCTCAACGACTCCATTGCCGAGGAGCTGAACAAGAAGTTGGCCAACAAGGTCGTGTACAACGTGGGACTCTGCATTTGTCTGTTTGATATCACCAAACTGGAGGATGCCTATGTATTCCCTGGGGATGGCGCATCACACACCAAAGTCCATTTTCGCTGCGTGGTGTTTCATCCATTCCTAGATGAGATTCTCATTGGGAAGATCAAAGGCTGCAGCCCAGAAGGAGTGCACGTCTCTCTAGGCTTCTTCGATGACATTCTCATCCCCCCAGAGTCACTGCAGCAGCCAGCCAAGTTCGACGAAGCGGAGCAGGTGTGGGTGTGGGAGTACGAGACGGAGGAAGGAGCACACGACCTCTACATGGACACCGGCGAGGAGATCCGCTTCCGGGTGGTGGACGAGAGCTTTGTTGACACGTCCCCCACAGGGCCCAGCTCAGCAGATGCCACCACTTCCAGTGAGGAGCTGCCAAAGAAGGAGGCTCCGTACACGCTTGTGGGATCCATCAGTGAGCCAGGCCTGGGCCTTCTCTCCTGGTGGACCAGCAACTAG; ; Translation Sequence: MFVLVEMVDT VRIPPWQFER KLNDSIAEEL NKKLANKVVY NVGLCICLFD ITKLEDAYVF PGDGASHTKV HFRCVVFHPF LDEILIGKIK GCSPEGVHVS LGFFDDILIP PESLQQPAKF DEAEQVWVWE YETEEGAHDL YMDTGEEIRF RVVDESFVDT SPTGPSSADA TTSSEELPKK EAPYTLVGSI SEPGLGLLSW WTSN
• Sequence Length: 204

• Species: Human
• Chromosome Location: 22q13.2
• cDNA Size: 615bp
• Vector Description: This shuttle vector contains the complete ORF. It is inseted Nde I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.
• Vector: (puc19-derived cloning vector)
• Preparation Before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid.
• Preparation and Storage: Store the plasmid at -20 degree C.

Product Categories/Family for POLR3H cdna clone

Epigenomics (Transcription & Translation)

NCBI and Uniprot Product Information

• NCBI GI #: 24475814
• NCBI GeneID: 171568
• NCBI Accession #: NP_612211.1
• NCBI GenBank Nucleotide #: NM_138338.4
• UniProt Accession #: Q9Y535
• NCBI Official Full Name: DNA-directed RNA polymerase III subunit RPC8 isoform a
• NCBI Official Synonym Full Names: RNA polymerase III subunit H
• NCBI Official Symbol: POLR3H
• NCBI Official Synonym Symbols: RPC8; RPC22.9
• NCBI Protein Information: DNA-directed RNA polymerase III subunit RPC8
• UniProt Protein Name: DNA-directed RNA polymerase III subunit RPC8
• Protein Family: DNA-directed RNA polymerase
• UniProt Gene Name: POLR3H
• UniProt Synonym Gene Names: KIAA1665; RPC8; RNA polymerase III subunit C8; RPC22.9
• UniProt Entry Name: RPC8_HUMAN

Uniprot Description

POLR3H: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: nucleoplasm; centrosome; DNA-directed RNA polymerase III complex; intracellular membrane-bound organelle; cytosol

Molecular Function: DNA binding; DNA-directed RNA polymerase activity

Biological Process: transcription from RNA polymerase III promoter; transcription initiation from RNA polymerase III promoter; termination of RNA polymerase III transcription; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; positive regulation of interferon type I production; innate immune response; gene expression; defense response to virus; RNA elongation from RNA polymerase III promoter