POLR1A cdna clone

POLR1A cDNA Clone

Gene Names

POLR1A; A190; RPA1; RPO14; AFDCIN; RPA194; RPO1-4

Synonyms

POLR1A; POLR1A cDNA Clone; POLR1A cdna clone

Ordering

Sequence

atgttgatctccaagaacatgccctggcggcggctgcagggcatttccttcgggatgtattcggctgaagagctcaagaaattaagtgttaaatccattacgaaccctcgatacctggacagcctggggaacccatcggcaaacggcctgtacgatttagctttgggccctgcagattccaaagaggtgtgctccacctgcgtgcaggacttcagcaactgttctgggcacctgggccacattgagctcccactcacagtgtataaccctctcctcttcgataagctgtacctgctgcttcggggctcttgtttaaactgccacatgctgacttgtccccgggccgtgattcacctcttactctgccagctgagggttctggaagtcggggccctacaagcagtctacgagcttgagagaattctgaacaggtttctggaagaaaatcccgatccctctgcctctgaaattcgggaggaattagaacaatacacaactgaaattgtgcagaacaacctcctggggtcccagggcgcacatgtaaagaacgtgtgtgagagcaagagcaagctcattgctctcttctggaaggcacatatgaatgctaagcgctgtccccactgcaagaccgggcgatccgttgtccgaaaggaacacaacagcaagttgactatcacgtttccagccatggtgcacaggacagctggccagaaggactctgagcccctgggaattgaggaagctcagataggaaaacgaggatacttaacacccaccagtgcccgcgaacacctttctgccctgtggaagaatgaaggattctttctgaactaccttttttcgggaatggatgatgatggtatggaatccagattcaatcccagtgtgttctttctagatttcttggtggtgccgccctcaaggtatcgcccagtcagtcgcctaggagaccagatgtttactaatggccagacggtgaacttgcaggctgtcatgaaggatgtagttctgattcgaaaacttctggcattgatggcccaagaacagaagttgccagaggaagtggccacacccactacagatgaggaaaaagactctttgattgctattgaccgatcctttttgagtacacttccaggccagtccctcatagacaaactttacaacatttggattcgccttcagagccacgtcaatattgtgtttgatagcgagatggacaaactaatgatggacaagtacccaggcattaggcagatcctggagaagaaagaaggcctgttccgaaaacacatgatgggaaagcgagtggactacgctgcgcgctcagtcatctgcccagacatgtacatcaacaccaacgaaattggaattcccatggtgtttgccacaaaactgacctacccacagccagttaccccatggaatgttcaggaacttaggcaagcggtcatcaacggccctaatgtgcacccaggagcctccatggtcatcaatgaggacggcagccgcacagccctgagcgctgtggacatgacccagcgagaggccgtggccaagcagcttctgaccccagccacgggggcacctaagccccaggggacaaaaattgtgtgccggcatgtgaagaatggggacattctgctactgaaccgacagcccacactgcacagaccctccatccaggcccaccgtgcccgcatcctgcctgaagagaaagtgctgcggctccactatgccaactgcaaggcctataatgccgactttgatggagacgagatgaatgcccatttcccccagagtgagctgggccgggccgaggcctacgtcctggcctgcactgatcagcagtaccttgttcccaaggatggccaaccattggcgggactgatccaggatcacatggtttcaggggcaagcatgactactcggggttgctttttcacccgggagcactatatggagctggtgtaccgaggactcacggacaaagtggggcgcgtgaagctcctttctccttccatcctgaagccctttccgctgtggacaggaaaacaggttgtgtcaacgctgctcataaatataatcccagaggaccacatcccactgaacttatctggaaaggcgaaaatcactgggaaagcctgggtgaaggaaactcctcgatccgttcctggctttaaccctgactcgatgtgcgagtcccaggtgatcatcagggaaggggagctgctctgcggagtgctggacaaggcgcactatgggagctccgcctacggcctggtccactgctgctatgagatctatggaggcgagaccagcggcaaggttctaacctgcctggcccgcctcttcaccgcctacctgcagctctacagaggcttcaccttgggcgtggaagacattttggtgaagccaaaggcagatgtcaagaggcaacgtatcattgaagaatccacccactgcgggccccaggctgtcagggctgcattaaacctgccagaagccgcatcatatgatgaggtccgaggaaaatggcaggatgcccatctgggcaaggaccagagggattttaacatgattgatctgaagttcaaggaggaagtgaaccattacagcaatgagattaacaaggcatgcatgccttttggcctacacagacagttcccagagaacagcctgcagatgatggtgcagtcgggagccaaaggttcaactgtgaacacgatgcagatctcgtgcctgctgggccagattgaactggaaggtcggagacccccgctgatggcgtctggcaagtcactgccctgctttgagccttatgagttcacccccagggctggtggctttgtcactggcaggttccttaccggcatcaaacctcctgagttcttcttccactgcatggcaggacgagagggcctggtggacactgctgtgaaaaccagccgctcaggctatctccaaaggtgcatcatcaagcacctagaggggctggtcgtgcagtatgatctcacggtccgtgacagtgacggcagtgtggtgcagttcctgtatggggaggatggcctggacatccccaagacacagttcctgcagcccaagcagttccccttcctggccagcaactacgaggtgataatgaaatcacagcatctccatgaagttttatccagagcagatcccaaaaaagctctccaccacttcagagctatcaaaaaatggcaaagcaagcaccccaacaccctgctgagaagaggcgccttcttgagttattcccagaaaattcaggaagctgtgaaagccctgaaacttgagagtgaaaaccgcaatggccgcagccctgggactcaggagatgctgaggatgtggtatgagttggatgaggaaagccgaaggaaataccagaagaaggcggccgcttgtcctgaccccagtctgtctgtctggcgtcctgacatctactttgcatcagtgtcagaaacatttgaaacaaaggttgatgactacagtcaagagtgggcagctcaaacagagaagagttatgagaaatcagagctttctctcgacaggttgaggaccttgctgcagctgaagtggcagcgctcactgtgtgagccgggcgaggctgtgggcctgctggctgcccagagcatcggagagccctccacccagatgaccctcaacaccttccactttgcaggcagaggcgagatgaacgtcaccctgggcattccaaggttgcgggagattctcatggtggccagcgccaacatcaagacacccatgatgagcgtgcccgtgctcaacaccaagaaagccctgaagagagtgaaaagcctgaagaagcaactcaccagggtgtgcttgggggaggtgttgcagaaaattgacgtccaggagtccttctgtatggaagaaaaacagaacaaattccaggtgtaccagctgcggtttcagttcctgccacatgcatattaccagcaggagaagtgcctgagacccgaggacatcctgcgcttcatggaaacaagattctttaaacttctgatggaatccatcaaaaagaagaataataaagcatcagctttcaggaacgtaaacactcgaagagctacacagcgggatctggacaacgctggggagttggggaggagtcggggagagcaggagggtgatgaggaagaggaggggcacattgtggatgctgaagctgaggagggagacgccgatgcctctgatgccaaacgcaaggagaagcaggaggaggaggttgattatgagagtgaggaagaggaggagagggagggcgaggagaacgacgatgaagacatgcaggaggaacgaaatccccacagggaaggtgctcgaaagacccaagagcaagatgaagaggtgggcttaggcactgaggaggacccgtcccttcccgccctcctgacgcagccccggaaacccacccacagccaggagccccaggggcccgaggccatggagcgccgggtccaggctgtgcgtgagatccacccgttcatagatgactaccagtacgacaccgaggagagcctgtggtgccaggtgacagtgaagctccctctgatgaagatcaactttgacatgagctccctggtagtatctttggcccatggtgccgtcatctatgcgaccaagggcatcactcggtgcctcctgaatgaaacaaccaacaataagaacgagaaggagcttgtgctaaacacagaaggaatcaacctcccagagctattcaagtatgcagaggtcctggatctgcgccgcctctactccaacgacatccacgccatagccaacacgtatggcattgaggccgcgctgcgggtgatcgagaaggagatcaaggatgtgtttgccgtgtatggcatcgcggtcgaccctcgccatctctccctggttgctgattatatgtgcttcgagggtgtttacaagccactgaatcgctttgggatccggtcaaactcttccccgctacagcagatgacatttgaaaccagcttccagtttctgaagcaagccaccatgctgggatcccacgatgagctgaggtctccttctgcctgccttgtggtcgggaaggtcgtcaggggcgggacaggcctgttcgagctcaagcagcctctgagatag

Sequence Length

5163

Vector

Please Inquire

NCBI and Uniprot Product Information

NCBI GI #

111599663

NCBI GeneID

25885

Molecular Weight

194,811 Da

NCBI Official Full Name

Homo sapiens polymerase (RNA) I polypeptide A, 194kDa, mRNA

NCBI Official Synonym Full Names

RNA polymerase I subunit A

NCBI Official Symbol

POLR1A

NCBI Official Synonym Symbols

A190; RPA1; RPO14; AFDCIN; RPA194; RPO1-4

NCBI Protein Information

DNA-directed RNA polymerase I subunit RPA1

UniProt Protein Name

DNA-directed RNA polymerase I subunit RPA1

UniProt Gene Name

POLR1A

UniProt Synonym Gene Names

RNA polymerase I subunit A1; RPA194

UniProt Entry Name

RPA1_HUMAN

NCBI Description

The protein encoded by this gene is the largest subunit of the RNA polymerase I complex. The encoded protein represents the catalytic subunit of the complex, which transcribes DNA into ribosomal RNA precursors. Defects in this gene are a cause of the Cincinnati type of acrofacial dysostosis. [provided by RefSeq, May 2016]

Uniprot Description

POLR1A: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. Belongs to the RNA polymerase beta' chain family.

Protein type: Transcription initiation complex; Nucleolus; EC 2.7.7.6; Transferase; Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 2p11.2

Cellular Component: cytoplasm; DNA-directed RNA polymerase I complex; nucleolus; nucleoplasm; nucleus

Molecular Function: protein binding

Biological Process: positive regulation of gene expression, epigenetic; RNA elongation from RNA polymerase I promoter; termination of RNA polymerase I transcription; transcription initiation from RNA polymerase I promoter

Disease: Acrofacial Dysostosis, Cincinnati Type

Research Articles on POLR1A

1. Consistent with this observation, we discovered that polr1a mutant zebrafish exhibited cranioskeletal anomalies mimicking the human phenotype. polr1a loss of function led to perturbed ribosome biogenesis and p53-dependent cell death

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Product Notes

The POLR1A polr1a (Catalog #AAA1269025) is a cDNA Clone and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: atgttgatct ccaagaacat gccctggcgg cggctgcagg gcatttcctt cgggatgtat tcggctgaag agctcaagaa attaagtgtt aaatccatta cgaaccctcg atacctggac agcctgggga acccatcggc aaacggcctg tacgatttag ctttgggccc tgcagattcc aaagaggtgt gctccacctg cgtgcaggac ttcagcaact gttctgggca cctgggccac attgagctcc cactcacagt gtataaccct ctcctcttcg ataagctgta cctgctgctt cggggctctt gtttaaactg ccacatgctg acttgtcccc gggccgtgat tcacctctta ctctgccagc tgagggttct ggaagtcggg gccctacaag cagtctacga gcttgagaga attctgaaca ggtttctgga agaaaatccc gatccctctg cctctgaaat tcgggaggaa ttagaacaat acacaactga aattgtgcag aacaacctcc tggggtccca gggcgcacat gtaaagaacg tgtgtgagag caagagcaag ctcattgctc tcttctggaa ggcacatatg aatgctaagc gctgtcccca ctgcaagacc gggcgatccg ttgtccgaaa ggaacacaac agcaagttga ctatcacgtt tccagccatg gtgcacagga cagctggcca gaaggactct gagcccctgg gaattgagga agctcagata ggaaaacgag gatacttaac acccaccagt gcccgcgaac acctttctgc cctgtggaag aatgaaggat tctttctgaa ctaccttttt tcgggaatgg atgatgatgg tatggaatcc agattcaatc ccagtgtgtt ctttctagat ttcttggtgg tgccgccctc aaggtatcgc ccagtcagtc gcctaggaga ccagatgttt actaatggcc agacggtgaa cttgcaggct gtcatgaagg atgtagttct gattcgaaaa cttctggcat tgatggccca agaacagaag ttgccagagg aagtggccac acccactaca gatgaggaaa aagactcttt gattgctatt gaccgatcct ttttgagtac acttccaggc cagtccctca tagacaaact ttacaacatt tggattcgcc ttcagagcca cgtcaatatt gtgtttgata gcgagatgga caaactaatg atggacaagt acccaggcat taggcagatc ctggagaaga aagaaggcct gttccgaaaa cacatgatgg gaaagcgagt ggactacgct gcgcgctcag tcatctgccc agacatgtac atcaacacca acgaaattgg aattcccatg gtgtttgcca caaaactgac ctacccacag ccagttaccc catggaatgt tcaggaactt aggcaagcgg tcatcaacgg ccctaatgtg cacccaggag cctccatggt catcaatgag gacggcagcc gcacagccct gagcgctgtg gacatgaccc agcgagaggc cgtggccaag cagcttctga ccccagccac gggggcacct aagccccagg ggacaaaaat tgtgtgccgg catgtgaaga atggggacat tctgctactg aaccgacagc ccacactgca cagaccctcc atccaggccc accgtgcccg catcctgcct gaagagaaag tgctgcggct ccactatgcc aactgcaagg cctataatgc cgactttgat ggagacgaga tgaatgccca tttcccccag agtgagctgg gccgggccga ggcctacgtc ctggcctgca ctgatcagca gtaccttgtt cccaaggatg gccaaccatt ggcgggactg atccaggatc acatggtttc aggggcaagc atgactactc ggggttgctt tttcacccgg gagcactata tggagctggt gtaccgagga ctcacggaca aagtggggcg cgtgaagctc ctttctcctt ccatcctgaa gccctttccg ctgtggacag gaaaacaggt tgtgtcaacg ctgctcataa atataatccc agaggaccac atcccactga acttatctgg aaaggcgaaa atcactggga aagcctgggt gaaggaaact cctcgatccg ttcctggctt taaccctgac tcgatgtgcg agtcccaggt gatcatcagg gaaggggagc tgctctgcgg agtgctggac aaggcgcact atgggagctc cgcctacggc ctggtccact gctgctatga gatctatgga ggcgagacca gcggcaaggt tctaacctgc ctggcccgcc tcttcaccgc ctacctgcag ctctacagag gcttcacctt gggcgtggaa gacattttgg tgaagccaaa ggcagatgtc aagaggcaac gtatcattga agaatccacc cactgcgggc cccaggctgt cagggctgca ttaaacctgc cagaagccgc atcatatgat gaggtccgag gaaaatggca ggatgcccat ctgggcaagg accagaggga ttttaacatg attgatctga agttcaagga ggaagtgaac cattacagca atgagattaa caaggcatgc atgccttttg gcctacacag acagttccca gagaacagcc tgcagatgat ggtgcagtcg ggagccaaag gttcaactgt gaacacgatg cagatctcgt gcctgctggg ccagattgaa ctggaaggtc ggagaccccc gctgatggcg tctggcaagt cactgccctg ctttgagcct tatgagttca cccccagggc tggtggcttt gtcactggca ggttccttac cggcatcaaa cctcctgagt tcttcttcca ctgcatggca ggacgagagg gcctggtgga cactgctgtg aaaaccagcc gctcaggcta tctccaaagg tgcatcatca agcacctaga ggggctggtc gtgcagtatg atctcacggt ccgtgacagt gacggcagtg tggtgcagtt cctgtatggg gaggatggcc tggacatccc caagacacag ttcctgcagc ccaagcagtt ccccttcctg gccagcaact acgaggtgat aatgaaatca cagcatctcc atgaagtttt atccagagca gatcccaaaa aagctctcca ccacttcaga gctatcaaaa aatggcaaag caagcacccc aacaccctgc tgagaagagg cgccttcttg agttattccc agaaaattca ggaagctgtg aaagccctga aacttgagag tgaaaaccgc aatggccgca gccctgggac tcaggagatg ctgaggatgt ggtatgagtt ggatgaggaa agccgaagga aataccagaa gaaggcggcc gcttgtcctg accccagtct gtctgtctgg cgtcctgaca tctactttgc atcagtgtca gaaacatttg aaacaaaggt tgatgactac agtcaagagt gggcagctca aacagagaag agttatgaga aatcagagct ttctctcgac aggttgagga ccttgctgca gctgaagtgg cagcgctcac tgtgtgagcc gggcgaggct gtgggcctgc tggctgccca gagcatcgga gagccctcca cccagatgac cctcaacacc ttccactttg caggcagagg cgagatgaac gtcaccctgg gcattccaag gttgcgggag attctcatgg tggccagcgc caacatcaag acacccatga tgagcgtgcc cgtgctcaac accaagaaag ccctgaagag agtgaaaagc ctgaagaagc aactcaccag ggtgtgcttg ggggaggtgt tgcagaaaat tgacgtccag gagtccttct gtatggaaga aaaacagaac aaattccagg tgtaccagct gcggtttcag ttcctgccac atgcatatta ccagcaggag aagtgcctga gacccgagga catcctgcgc ttcatggaaa caagattctt taaacttctg atggaatcca tcaaaaagaa gaataataaa gcatcagctt tcaggaacgt aaacactcga agagctacac agcgggatct ggacaacgct ggggagttgg ggaggagtcg gggagagcag gagggtgatg aggaagagga ggggcacatt gtggatgctg aagctgagga gggagacgcc gatgcctctg atgccaaacg caaggagaag caggaggagg aggttgatta tgagagtgag gaagaggagg agagggaggg cgaggagaac gacgatgaag acatgcagga ggaacgaaat ccccacaggg aaggtgctcg aaagacccaa gagcaagatg aagaggtggg cttaggcact gaggaggacc cgtcccttcc cgccctcctg acgcagcccc ggaaacccac ccacagccag gagccccagg ggcccgaggc catggagcgc cgggtccagg ctgtgcgtga gatccacccg ttcatagatg actaccagta cgacaccgag gagagcctgt ggtgccaggt gacagtgaag ctccctctga tgaagatcaa ctttgacatg agctccctgg tagtatcttt ggcccatggt gccgtcatct atgcgaccaa gggcatcact cggtgcctcc tgaatgaaac aaccaacaat aagaacgaga aggagcttgt gctaaacaca gaaggaatca acctcccaga gctattcaag tatgcagagg tcctggatct gcgccgcctc tactccaacg acatccacgc catagccaac acgtatggca ttgaggccgc gctgcgggtg atcgagaagg agatcaagga tgtgtttgcc gtgtatggca tcgcggtcga ccctcgccat ctctccctgg ttgctgatta tatgtgcttc gagggtgttt acaagccact gaatcgcttt gggatccggt caaactcttc cccgctacag cagatgacat ttgaaaccag cttccagttt ctgaagcaag ccaccatgct gggatcccac gatgagctga ggtctccttc tgcctgcctt gtggtcggga aggtcgtcag gggcgggaca ggcctgttcg agctcaagca gcctctgaga tag. It is sometimes possible for the material contained within the vial of "POLR1A, cDNA Clone" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

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Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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POLR1A cdna clone