IDH1 cdna clone
IDH1 cDNA Clone
NCBI and Uniprot Product Information
NCBI Description
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013]
Uniprot Description
IDH1: an oxidoreductase that catalyzes the third step of the TCA cycle: the oxidative decarboxylation of isocitrate, consuming NADP(+), and producing alpha-ketoglutarate (alpha-KG) and CO2. Alpha-KG is an activator the dioxygenases that hydroxylate the transcription factor HIF and lead to its degradation by VHL. Since HIF turns on oncogenic pathways, IDH1 has apparent tumor suppressor activity. Homo-dimerization is required for activity. Each subunit binds 1 magnesium or manganese ion. IDH1 is located in the cytosol and peroxisomes. Somatic mutations affecting arginine 132 (R132) are found in 80% of grade II?III gliomas and secondary glioblastomas in humans, and cause disease in a tissue-specific fashion. Only a single copy of the gene has been found to be mutated in tumours. Mutations of R132 to H, C, S, G, V, or L have been reported to be neomorphic, abolishing the conversion of isocitrate to alpha-KG. Instead, alpha-KG is converted to R(-)-2-hydroxyglutarate (2HG). Elevated levels of 2HG correlate with an elevated risk of malignant brain tumors. Neomorphic mutations in IDH1 and IDH2 convert alpha-KG to 2-hydroxyglutarate (2-HG) occur in a high percentage of patients with cytogenetically normal acute myeloid leukemia (AML).
Protein type: Oxidoreductase; EC 1.1.1.42; Carbohydrate Metabolism - citrate (TCA) cycle; Other Amino Acids Metabolism - glutathione
Chromosomal Location of Human Ortholog: 2q33.3
Cellular Component: cell-cell adherens junction; cytoplasm; cytosol; peroxisomal matrix; peroxisome
Molecular Function: (R)-2-hydroxyglutarate dehydrogenase activity; isocitrate dehydrogenase (NADP+) activity; magnesium ion binding; protein homodimerization activity; receptor binding
Biological Process: 2-oxoglutarate metabolic process; isocitrate metabolic process; NADPH regeneration
Disease: Glioma Susceptibility 1
Research Articles on IDH1
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Product Notes
The IDH1 idh1 (Catalog #AAA1271096) is a cDNA Clone and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: atgtccaaaa aaatcagtgg cggttctgtg gtagagatgc aaggagatga aatgacacga atcatttggg aattgattaa agagaaactc atttttccct acgtggaatt ggatctacat agctatgatt taggcataga gaatcgtgat gccaccaacg accaagtcac caaggatgct gcagaagcta taaagaagca taatgttggc gtcaaatgtg ccactatcac tcctgatgag aagagggttg aggagttcaa gttgaaacaa atgtggaaat caccaaatgg caccatacga aatattctgg gtggcacggt cttcagagaa gccattatct gcaaaaatat cccccggctt gtgagtggat gggtaaaacc tatcatcata ggtcgtcatg cttatgggga tcaatacaga gcaactgatt ttgttgttcc tgggcctgga aaagtagaga taacctacac accaagtgac ggaacccaaa aggtgacata cctggtacat aactttgaag aaggtggtgg tgttgccatg gggatgtata atcaagataa gtcaattgaa gattttgcac acagttcctt ccaaatggct ctgtctaagg gttggccttt gtatctgagc accaaaaaca ctattctgaa gaaatatgat gggcgtttta aagacatctt tcaggagata tatgacaagc agtacaagtc ccagtttgaa gctcaaaaga tctggtatga gcataggctc atcgacgaca tggtggccca agctatgaaa tcagagggag gcttcatctg ggcctgtaaa aactatgatg gtgacgtgca gtcggactct gtggcccaag ggtatggctc tctcggcatg atgaccagcg tgctggtttg tccagatggc aagacagtag aagcagaggc tgcccacggg actgtaaccc gtcactaccg catgtaccag aaaggacagg agacgtccac caatcccatt gcttccattt ttgcctggac cagagggtta gcccacagag caaagcttga taacaataaa gagcttgcct tctttgcaaa tgctttggaa gaagtctcta ttgagacaat tgaggctggc ttcatgacca aggacttggc tgcttgcatt aaaggtttac ccaatgtgca acgttctgac tacttgaata catttgagtt catggataaa cttggagaaa acttgaagat caaactagct caggccaaac tttaa. It is sometimes possible for the material contained within the vial of "IDH1, cDNA Clone" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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