Rabbit anti-Human GPRC6A Polyclonal Antibody | anti-GPRC6A antibody

GPRC6A, ID (GPRC6A, G-protein coupled receptor family C group 6 member A, G-protein coupled receptor GPCR33) (Biotin)

Cat. Num. AAA6304131

• Gene Names: GPRC6A; GPCR; bA86F4.3
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GPRC6A; Polyclonal Antibody; ID (GPRC6A; G-protein coupled receptor family C group 6 member A; G-protein coupled receptor GPCR33) (Biotin); G-protein coupled receptor GPCR33; anti-GPRC6A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-GPRC6A antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GPRC6A antibody is generated from rabbits immunized with a KLH conjugated synthetic between 488-517 amino acids selected from the Central region of human GPRC6A.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GPRC6A Antibody (Center) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the GPRC6A antibody detected the GPRC6A protein (arrow).)

Related Product Information for anti-GPRC6A antibody

Members of family C of the G protein-coupled receptor (GPCR) superfamily, such as GPRC6A, are characterized by an evolutionarily conserved amino acid-sensing motif linked to an intramembranous 7-transmembrane loop region. Several members of GPCR family C, including GPRC6A, also have a long N-terminal domain (summary by Pi et al., 2005 [PubMed 16199532]).

Product Categories/Family for anti-GPRC6A antibody

Antibodies; G Protein Receptors

References

Giroux, S., et al. Bone 47(5):975-981(2010). Takata, R., et al. Nat. Genet. 42(9):751-754(2010). Pi, M., et al. J. Bone Miner. Res. 25(5):1092-1102(2010). Dowal, L., et al. J. Biol. Chem. 281(33):23999-24014(2006). Pi, M., et al. J. Biol. Chem. 280(48):40201-40209(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 112807234
• NCBI GeneID: 222545
• NCBI Accession #: NP_683766.2
• NCBI GenBank Nucleotide #: NM_148963.2
• UniProt Accession #: Q5T6X5; Q6JK43; Q6JK44; Q8NGU8; Q8NHZ9; Q8TDT6
• Molecular Weight: 104,753 Da
• NCBI Official Full Name: G-protein coupled receptor family C group 6 member A
• NCBI Official Synonym Full Names: G protein-coupled receptor, family C, group 6, member A
• NCBI Official Symbol: GPRC6A
• NCBI Official Synonym Symbols: GPCR; bA86F4.3
• NCBI Protein Information: G-protein coupled receptor family C group 6 member A; hGPCR33; hGPRC6A; predicted with SOSUI analysis; G-protein coupled receptor GPCR33; seven transmembrane helix receptor
• UniProt Protein Name: G-protein coupled receptor family C group 6 member A
• Protein Family: G-protein coupled receptor family
• UniProt Gene Name: GPRC6A
• UniProt Synonym Gene Names: hGPRC6A; hGPCR33
• UniProt Entry Name: GPC6A_HUMAN

NCBI Description

Members of family C of the G protein-coupled receptor (GPCR) superfamily, such as GPRC6A, are characterized by an evolutionarily conserved amino acid-sensing motif linked to an intramembranous 7-transmembrane loop region. Several members of GPCR family C, including GPRC6A, also have a long N-terminal domain (summary by Pi et al., 2005 [PubMed 16199532]).[supplied by OMIM, Nov 2010]

Uniprot Description

GPRC6A: Receptor activated by amino acids with a preference for basic amino acids such as L-Lys, L-Arg and L-ornithine but also by small and polar amino acids. The L-alpha amino acids respond is augmented by divalent cations Ca(2+) and Mg(2+). Activated by extracellular calcium and osteocalin. Seems to act through a G(q)/G(11) and G(i)-coupled pathway. Mediates the non-genomic effects of androgens in multiple tissue. May coordinates nutritional and hormonal anabolic signals through the sensing of extracellular amino acids, osteocalcin, divalents ions and its responsiveness to anabolic steroids. Belongs to the G-protein coupled receptor 3 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; Membrane protein, integral; GPCR, family 3; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 6q22.1

Cellular Component: cell surface; integral to membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; calcium-mediated signaling; response to amino acid stimulus