DPM1 cdna clone

DPM1

Cat. Num. AAA200579

• Gene Names: DPM1; MPDS; CDGIE
• Synonyms: DPM1; CDGIE; MPDS; DPM1 cdna clone

• Form/Format: Lyophilized
• Sequence: Nucleotide Sequence: ATGGCCTCCT TGGAAGTCAG TCGTAGTCCT CGCAGGTCTC GGCGGGAGCT GGAAGTGCGC AGTCCACGAC AGAACAAATA TTCGGTGCTT TTACCTACCT ACAACGAGCG CGAGAACCTG CCGCTCATCG TGTGGCTGCT GGTGAAAAGC TTCTCCGAGA GTGGAATCAA CTATGAAATT ATAATCATAG ATGATGGAAG CCCAGATGGA ACAAGGGATG TTGCTGAACA GTTGGAGAAG ATCTATGGGT CAGACAGAAT TCTTCTAAGA CCACGAGAGA AAAAGTTGGG ACTAGGAACT GCATATATTC ATGGAATGAA ACATGCCACA GGAAACTACA TCATTATTAT GGATGCTGAT CTCTCACACC ATCCAAAATT TATTCCTGAA TTTATTAGGA AGCAAAAGGA GGGTAATTTT GATATTGTCT CTGGAACTCG CTACAAAGGA AATGGAGGTG TATATGGCTG GGATTTGAAA AGAAAAATAA TCAGCCGTGG GGCCAATTTT TTAACTCAGA TCTTGCTGAG ACCAGGAGCA TCTGATTTAA CAGGAAGTTT CAGATTATAC CGAAAAGAAG TTCTAGAGAA ATTAATAGAA AAATGTGTTT CTAAAGGCTA CGTCTTCCAG ATGGAGATGA TTGTTCGGGC AAGACAGTTG AATTATACTA TTGGCGAGGT TCCAATATCA TTTGTGGATC GTGTTTATGG TGAATCCAAG TTGGGAGGAA ATGAAATAGT ATCTTTCTTG AAAGGATTAT TGACTCTTTT TGCTACTACA TAA; ; Translation Sequence: MASLEVSRSP RRSRRELEVR SPRQNKYSVL LPTYNERENL PLIVWLLVKS FSESGINYEI IIIDDGSPDG TRDVAEQLEK IYGSDRILLR PREKKLGLGT AYIHGMKHAT GNYIIIMDAD LSHHPKFIPE FIRKQKEGNF DIVSGTRYKG NGGVYGWDLK RKIISRGANF LTQILLRPGA SDLTGSFRLY RKEVLEKLIE KCVSKGYVFQ MEMIVRARQL NYTIGEVPIS FVDRVYGESK LGGNEIVSFL KGLLTLFATT
• Sequence Length: 260

• Species: Human
• Chromosome Location: 20q13.13
• OMIM Reference Number: 603503
• cDNA Size: 783bp
• Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.
• Vector: (puc19-derived cloning vector)
• Preparation Before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid.
• Preparation and Storage: Store the plasmid at -20 degree C.

Related Product Information for DPM1 cdna clone

Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. Human DPM1 lacks a carboxy-terminal transmembrane domain and signal sequence and is regulated by DPM2.

Product Categories/Family for DPM1 cdna clone

Tags & Cell Markers

NCBI and Uniprot Product Information

• NCBI GI #: 4503363
• NCBI GeneID: 8813
• NCBI Accession #: NP_003850.1
• NCBI GenBank Nucleotide #: NM_003859.1
• UniProt Accession #: O60762
• NCBI Official Full Name: dolichol-phosphate mannosyltransferase subunit 1 isoform 3
• NCBI Official Synonym Full Names: dolichyl-phosphate mannosyltransferase subunit 1, catalytic
• NCBI Official Symbol: DPM1
• NCBI Official Synonym Symbols: MPDS; CDGIE
• NCBI Protein Information: dolichol-phosphate mannosyltransferase subunit 1
• UniProt Protein Name: Dolichol-phosphate mannosyltransferase subunit 1
• Protein Family: Dolichol-phosphate mannosyltransferase
• UniProt Gene Name: DPM1
• UniProt Synonym Gene Names: DPM synthase subunit 1; MPD synthase subunit 1
• UniProt Entry Name: DPM1_HUMAN

NCBI Description

Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. Human DPM1 lacks a carboxy-terminal transmembrane domain and signal sequence and is regulated by DPM2. Mutations in this gene are associated with congenital disorder of glycosylation type Ie. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2015]

Uniprot Description

DPM1: Transfers mannose from GDP-mannose to dolichol monophosphate to form dolichol phosphate mannose (Dol-P-Man) which is the mannosyl donor in pathways leading to N-glycosylation, glycosyl phosphatidylinositol membrane anchoring, and O- mannosylation of proteins. Defects in DPM1 are the cause of congenital disorder of glycosylation type 1E (CDG1E). CDGs are metabolic deficiencies in glycoprotein biosynthesis that usually cause severe mental and psychomotor retardation. They are characterized by under-glycosylated serum glycoproteins. CDG1E is an autosomal recessive disorder, characterized by severe developmental delay, hypotnia, seizures, and dysmorphic features. Belongs to the glycosyltransferase 2 family.

Protein type: Transferase; Glycan Metabolism - N-glycan biosynthesis; EC 2.4.1.83

Chromosomal Location of Human Ortholog: 20q13.13

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; nucleus

Molecular Function: mannose binding; protein binding; alcohol binding; dolichyl-phosphate-mannose-protein mannosyltransferase activity; dolichyl-phosphate beta-D-mannosyltransferase activity

Biological Process: cellular protein metabolic process; dolichol-linked oligosaccharide biosynthetic process; protein amino acid mannosylation; protein amino acid O-linked mannosylation; GPI anchor biosynthetic process; GDP-mannose metabolic process; protein amino acid N-linked glycosylation via asparagine; dolichol metabolic process; C-terminal protein lipidation; post-translational protein modification

Disease: Congenital Disorder Of Glycosylation, Type Ie