ARG1 cdna clone

ARG1

Cat. Num. AAA200527

• Synonyms: ARG1; ARG1 cdna clone

• Form/Format: Lyophilized
• Sequence: Nucleotide Sequence: ATGAGCGCCA AGTCCAGAAC CATAGGGATT ATTGGAGCTC CTTTCTCAAA GGGACAGCCA CGAGGAGGGG TGGAAGAAGG CCCTACAGTA TTGAGAAAGG CTGGTCTGCT TGAGAAACTT AAAGAACAAG AGTGTGATGT GAAGGATTAT GGGGACCTGC CCTTTGCTGA CATCCCTAAT GACAGTCCCT TTCAAATTGT GAAGAATCCA AGGTCTGTGG GAAAAGCAAG CGAGCAGCTG GCTGGCAAGG TGGCAGAAGT CAAGAAGAAC GGAAGAATCA GCCTGGTGCT GGGCGGAGAC CACAGTTTGG CAATTGGAAG CATCTCTGGC CATGCCAGGG TCCACCCTGA TCTTGGAGTC ATCTGGGTGG ATGCTCACAC TGATATCAAC ACTCCACTGA CAACCACAAG TGGAAACTTG CATGGACAAC CTGTATCTTT CCTCCTGAAG GAACTAAAAG GAAAGATTCC CGATGTGCCA GGATTCTCCT GGGTGACTCC CTGTATATCT GCCAAGGATA TTGTGTATAT TGGCTTGAGA GACGTGGACC CTGGGGAACA CTACATTTTG AAAACTCTAG GCATTAAATA CTTTTCAATG ACTGAAGTGG ACAGACTAGG AATTGGCAAG GTGATGGAAG AAACACTCAG CTATCTACTA GGAAGAAAGA AAAGGCCAAT TCATCTAAGT TTTGATGTTG ACGGACTGGA CCCATCTTTC ACACCAGCTA CTGGCACACC AGTCGTGGGA GGTCTGACAT ACAGAGAAGG TCTCTACATC ACAGAAGAAA TCTACAAAAC AGGGCTACTC TCAGGATTAG ATATAATGGA AGTGAACCCA TCCCTGGGGA AGACACCAGA AGAAGTAACT CGAACAGTGA ACACAGCAGT TGCAATAACC TTGGCTTGTT TCGGACTTGC TCGGGAGGGT AATCACAAGC CTATTGACTA CCTTAACCCA CCTAAGTAA; ; Translation Sequence: MSAKSRTIGI IGAPFSKGQP RGGVEEGPTV LRKAGLLEKL KEQECDVKDY GDLPFADIPN DSPFQIVKNP RSVGKASEQL AGKVAEVKKN GRISLVLGGD HSLAIGSISG HARVHPDLGV IWVDAHTDIN TPLTTTSGNL HGQPVSFLLK ELKGKIPDVP GFSWVTPCIS AKDIVYIGLR DVDPGEHYIL KTLGIKYFSM TEVDRLGIGK VMEETLSYLL GRKKRPIHLS FDVDGLDPSF TPATGTPVVG GLTYREGLYI TEEIYKTGLL SGLDIMEVNP SLGKTPEEVT RTVNTAVAIT LACFGLAREG NHKPIDYLNP PK
• Sequence Length: 322

• Species: Human
• Chromosome Location: 6q23
• OMIM Reference Number: 608313
• cDNA Size: 969bp
• Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.
• Vector: (puc19-derived cloning vector)
• Preparation Before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid.
• Preparation and Storage: Store the plasmid at -20 degree C.

Related Product Information for ARG1 cdna clone

Arginase is a manganese-containing enzyme which catalyzes the hydrolysis of arginine to ornithine and urea. It is the final enzyme of the urea cycle. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The type II isoform has been implicated in the regulation of the arginine/ornithine concentrations in the cell. It is located in mitochondria of several tissues in the body, with most abundance in the kidney and prostate.

Product Categories/Family for ARG1 cdna clone

Metabolism; Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 10947139
• NCBI GeneID: 383
• NCBI Accession #: NP_000036.2
• NCBI GenBank Nucleotide #: NM_000045.3
• UniProt Accession #: P05089
• NCBI Official Full Name: arginase-1 isoform 2
• NCBI Official Synonym Full Names: arginase 1
• NCBI Official Symbol: ARG1
• NCBI Protein Information: arginase-1
• UniProt Protein Name: Arginase-1
• Protein Family: Arginase
• UniProt Gene Name: ARG1
• UniProt Entry Name: ARGI1_HUMAN

NCBI Description

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

ARG1: Homotrimer. By arginine or homoarginine. Belongs to the arginase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - arginine and proline; Hydrolase; EC 3.5.3.1

Chromosomal Location of Human Ortholog: 6q23

Cellular Component: extracellular space; neuron projection; cell soma; cytoplasm; cytosol; nucleus

Molecular Function: manganese ion binding; arginase activity

Biological Process: response to drug; mammary gland involution; maternal process involved in pregnancy; response to herbicide; liver development; response to amino acid stimulus; response to vitamin A; response to manganese ion; response to selenium ion; response to vitamin E; response to cadmium ion; response to zinc ion; response to methylmercury; arginine catabolic process; positive regulation of endothelial cell proliferation; response to axon injury; collagen biosynthetic process; response to amine stimulus; urea cycle; lung development

Disease: Argininemia