APRT cdna clone

APRT

Cat. Num. AAA200526

• Gene Names: APRT; AMP; APRTD
• Synonyms: APRT; AMP; APRTD; APRT cdna clone

• Form/Format: Lyophilized
• Sequence: Nucleotide Sequence: ATGGCCGACT CCGAGCTGCA GCTGGTTGAG CAGCGGATCC GCAGCTTCCC CGACTTCCCC ACCCCAGGCG TGGTATTCAG GGACATCTCG CCCGTCCTGA AGGACCCCGC CTCCTTCCGC GCCGCCATCG GCCTCCTGGC GCGACACCTG AAGGCGACCC ACGGGGGCCG CATCGACTAC ATCGCAGGCC TAGACTCCCG AGGCTTCCTC TTTGGCCCCT CCCTGGCCCA GGAGCTTGGA CTGGGCTGCG TGCTCATCCG AAAGCGGGGG AAGCTGCCAG GCCCCACTCT GTGGGCCTCC TATTCCCTGG AGTACGGGAA GGCTGAGCTG GAGATTCAGA AAGACGCCCT GGAGCCAGGA CAGAGGGTGG TCGTCGTGGA TGATCTGCTG GCCACTGGTG GAACCATGAA CGCTGCCTGT GAGCTGCTGG GCCGCCTGCA GGCTGAGGTC CTGGAGTGCG TGAGCCTGGT GGAGCTGACC TCGCTTAAGG GCAGGGAGAA GCTGGCACCT GTACCCTTCT TCTCTCTCCT GCAGTATGAG TGA; ; Translation Sequence: MADSELQLVE QRIRSFPDFP TPGVVFRDIS PVLKDPASFR AAIGLLARHL KATHGGRIDY IAGLDSRGFL FGPSLAQELG LGCVLIRKRG KLPGPTLWAS YSLEYGKAEL EIQKDALEPG QRVVVVDDLL ATGGTMNAAC ELLGRLQAEV LECVSLVELT SLKGREKLAP VPFFSLLQYE
• Sequence Length: 180

• Species: Human
• Chromosome Location: 16q24
• OMIM Reference Number: 102600
• cDNA Size: 543bp
• Vector Description: This shuttle vector contains the complete ORF. It is inseted Nde I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.
• Vector: (puc19-derived cloning vector)
• Preparation Before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid.
• Preparation and Storage: Store the plasmid at -20 degree C.

Related Product Information for APRT cdna clone

APRT (adenine phosphoribosyltransferase) is a 180 amino acid protein that localizes to the cytoplasm and belongs to the purine/pyrimidine phosphoribosyltransferase family. Existing as a homodimer, APRT functions to catalyze the formation of inorganic pyrophosphate and AMP from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP), a reaction that is essential for both purine metabolism and AMP biosynthesis. It also produces adenine as a by-product of the polyamine biosynthesis pathway.

Product Categories/Family for APRT cdna clone

Metabolism; Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 4502171
• NCBI GeneID: 353
• NCBI Accession #: NP_000476.1
• NCBI GenBank Nucleotide #: NM_000485.2
• UniProt Accession #: P07741
• NCBI Official Full Name: adenine phosphoribosyltransferase isoform a
• NCBI Official Synonym Full Names: adenine phosphoribosyltransferase
• NCBI Official Symbol: APRT
• NCBI Official Synonym Symbols: AMP; APRTD
• NCBI Protein Information: adenine phosphoribosyltransferase
• UniProt Protein Name: Adenine phosphoribosyltransferase
• Protein Family: Probable adenine phosphoribosyltransferase
• UniProt Gene Name: APRT
• UniProt Synonym Gene Names: APRT
• UniProt Entry Name: APT_HUMAN

NCBI Description

Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

APRT: Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis. Defects in APRT are the cause of adenine phosphoribosyltransferase deficiency (APRTD); also known as 2,8-dihydroxyadenine urolithiasis. An enzymatic deficiency that can lead to urolithiasis and renal failure. Patients have 2,8-dihydroxyadenine (DHA) urinary stones. Belongs to the purine/pyrimidine phosphoribosyltransferase family.

Protein type: EC 2.4.2.7; Transferase; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 16q24

Cellular Component: nucleoplasm; cytoplasm; cytosol

Molecular Function: adenine binding; adenine phosphoribosyltransferase activity; AMP binding

Biological Process: lactation; grooming behavior; cellular response to insulin stimulus; nucleobase, nucleoside and nucleotide metabolic process; adenine salvage; purine salvage; purine base metabolic process

Disease: Adenine Phosphoribosyltransferase Deficiency