AES cdna clone

AES

Cat. Num. AAA200558

• Gene Names: TLE5; AES; GRG; ESP1; GRG5; AES-1; AES-2; Grg-5
• Synonyms: AES; AES-1; AES-2; ESP1; GRG; Grg-5; GRG5; TLE5; AES cdna clone

• Form/Format: Lyophilized
• Sequence: Nucleotide Sequence: ATGATGTTTC CACAAAGCAG GCATTCGGGC TCCTCGCACC TACCCCAGCA ACTCAAATTC ACCACCTCGG ACTCCTGCGA CCGCATCAAA GACGAATTTC AGCTACTGCA AGCTCAGTAC CACAGCCTCA AGCTCGAATG TGACAAGTTG GCCAGTGAGA AGTCAGAGAT GCAGCGTCAC TATGTGATGT ACTACGAGAT GTCCTACGGC TTGAACATCG AGATGCACAA ACAGGCTGAG ATCGTCAAAA GGCTGAACGG GATTTGTGCC CAGGTCCTGC CCTACCTCTC CCAAGAGCAC CAGCAGCAGG TCTTGGGAGC CATTGAGAGG GCCAAGCAGG TCACCGCTCC CGAGCTGAAC TCTATCATCC GACAGCAGCT CCAAGCCCAC CAGCTGTCCC AGCTGCAGGC CCTGGCCCTG CCCTTGACCC CACTACCCGT GGGGCTGCAG CCGCCTTCGC TGCCGGCGGT CAGCGCAGGC ACCGGCCTCC TCTCGCTGTC CGCGCTGGGT TCCCAGGCCC ACCTCTCCAA GGAAGACAAG AACGGGCACG ATGGTGACAC CCACCAGGAG GATGATGGCG AGAAGTCGGA TTAG; ; Translation Sequence: MMFPQSRHSG SSHLPQQLKF TTSDSCDRIK DEFQLLQAQY HSLKLECDKL ASEKSEMQRH YVMYYEMSYG LNIEMHKQAE IVKRLNGICA QVLPYLSQEH QQQVLGAIER AKQVTAPELN SIIRQQLQAH QLSQLQALAL PLTPLPVGLQ PPSLPAVSAG TGLLSLSALG SQAHLSKEDK NGHDGDTHQE DDGEKSD
• Sequence Length: 197

• Species: Human
• Chromosome Location: 19p13.3
• OMIM Reference Number: 600188
• cDNA Size: 594bp
• Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.
• Vector: (puc19-derived cloning vector)
• Preparation Before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid.
• Preparation and Storage: Store the plasmid at -20 degree C.

Related Product Information for AES cdna clone

Amino-terminal enhancer of split, also known AES, belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. This protein is expressed predominately in fetal brain, liver, lung, heart and kidney and in adult muscle. In addition, AES can repress NFkB-regulated gene expression and is thought to play an important role in initiating and maintaining cell differentiation events.

Product Categories/Family for AES cdna clone

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 5706731
• NCBI GeneID: 166
• NCBI Accession #: NP_001121.2
• NCBI GenBank Nucleotide #: NM_001130.5
• UniProt Accession #: Q08117
• NCBI Official Full Name: TLE family member 5 isoform b
• NCBI Official Synonym Full Names: TLE family member 5, transcriptional modulator
• NCBI Official Symbol: TLE5
• NCBI Official Synonym Symbols: AES; GRG; ESP1; GRG5; AES-1; AES-2; Grg-5
• NCBI Protein Information: TLE family member 5; amino-terminal enhancer of split
• UniProt Protein Name: Amino-terminal enhancer of split
• Protein Family: Acetyl esterase
• UniProt Gene Name: AES
• UniProt Synonym Gene Names: GRG; GRG5; Amino enhancer of split
• UniProt Entry Name: AES_HUMAN

NCBI Description

The protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The encoded protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

AES: Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Belongs to the WD repeat Groucho/TLE family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleus

Molecular Function: protein binding; transcription corepressor activity

Biological Process: organ morphogenesis; Wnt receptor signaling pathway; transcription, DNA-dependent; regulation of growth; multicellular organismal development; cellular response to extracellular stimulus; negative regulation of transcription from RNA polymerase II promoter; negative regulation of protein binding; negative regulation of transcription, DNA-dependent; skeletal development