Rat Cyclin Dependent Kinase Inhibitor 1A ELISA Kit | CDKN1A elisa kit

Rat Cyclin Dependent Kinase Inhibitor 1A ELISA Kit

Cat. Num. AAA760511

• Gene Names: CDKN1A; P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1
• Reactivity: Rat
• Synonyms: Cyclin Dependent Kinase Inhibitor 1A; Rat Cyclin Dependent Kinase Inhibitor 1A ELISA Kit; CDKN1A/CDKN1A/CIP1/CAP20cyclin-dependent kinase inhibitor 1/CDK-interacting protein 1/CDKN1melanoma differentiation associated protein 6/CIP1WAF1CDK-interaction protein 1/cyclin-dependent kinase inhibitor 1A (p21; Cip1)/MDA6/MDA-6/Melanoma differentiation-associated protein 6/p21/P21/p21CIP1/p21Cip1/Waf1/PIC1/SDI1DNA synthesis inhibitor/wild-type p53-activated fragment 1; CDKN1A elisa kit

• Reactivity: Rat
• Sequence Length: 164

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78.125-5000pg/ml
• Sensitivity: 46.875pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for CDKN1A elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Rat CDKN1A antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat CDKN1A antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat CDKN1A amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat CDKN1A can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 197692545
• NCBI GeneID: 1026
• Molecular Weight: 18,119 Da
• NCBI Official Full Name: cyclin-dependent kinase inhibitor 1A, partial
• NCBI Official Synonym Full Names: cyclin-dependent kinase inhibitor 1A
• NCBI Official Symbol: CDKN1A
• NCBI Official Synonym Symbols: P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1
• NCBI Protein Information: cyclin-dependent kinase inhibitor 1
• UniProt Protein Name: Cyclin-dependent kinase inhibitor 1
• UniProt Gene Name: CDKN1A
• UniProt Synonym Gene Names: CAP20; CDKN1; CIP1; MDA6; PIC1; SDI1; WAF1; MDA-6
• UniProt Entry Name: CDN1A_HUMAN

NCBI Description

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

p21Cip1: a cell-cycle regulatory protein that Interacts with cyclin-CDK2 and -CDK4, inhibiting cell cycle progression at G1. Its expression is tightly controlled by p53, through which this protein mediates the p53-dependent cell cycle arrest at G1 phase.

Protein type: Cell cycle regulation; Inhibitor

Chromosomal Location of Human Ortholog: 6p21.2

Cellular Component: cyclin-dependent protein kinase holoenzyme complex; cytosol; nucleoplasm; nucleus; perinuclear region of cytoplasm

Molecular Function: cyclin binding; cyclin-dependent protein kinase activating kinase activity; cyclin-dependent protein kinase inhibitor activity; metal ion binding; protein binding; protein complex binding; ubiquitin protein ligase binding

Biological Process: cell cycle arrest; cellular response to amino acid starvation; cellular response to extracellular stimulus; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; G1/S transition of mitotic cell cycle; G2/M transition of mitotic cell cycle; innate immune response; mitotic cell cycle; negative regulation of apoptosis; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of cyclin-dependent protein kinase activity; negative regulation of phosphorylation; nerve growth factor receptor signaling pathway; organ regeneration; phosphoinositide-mediated signaling; positive regulation of B cell proliferation; positive regulation of fibroblast proliferation; positive regulation of programmed cell death; Ras protein signal transduction; regulation of cyclin-dependent protein kinase activity; regulation of protein import into nucleus, translocation; response to arsenic; response to corticosterone stimulus; response to DNA damage stimulus; response to drug; response to hyperoxia; response to organic nitrogen; response to toxin; response to X-ray