Cyclin Dependent Kinase 9 Recombinant Protein | CDK9 recombinant protein

Recombinant Cyclin Dependent Kinase 9 (CDK9)

Cat. Num. AAA2030120

• Gene Names: CDK9; TAK; C-2k; CTK1; CDC2L4; PITALRE
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Cyclin Dependent Kinase 9; Recombinant Cyclin Dependent Kinase 9 (CDK9); CDK9 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 7.8 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- DFCEHDL AGLLSNVLVK FTLSEIKRVM QMLLNGLYYI HRNKILHRDM KAANVLITRD GVLKLADFGL ARAFSLAKNS QPNRYTNRVV TLWYRPPELL LGERDYGPPI DLWGAGCIMA EMWTRSPIMQ GNTEQHQLAL ISQLCGSITP EVWPNVDNYE LYEKLELVKG QKRKVKDRLK AYVRDPYALD LIDKLLVLDPAQRIDSDDAL NHDFF
• Sequence Length: 372

• Applicable Applications for CDK9 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 28.3kDa
• Accurate Molecular Mass (KD): 28kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Lys284~Val389
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4502747
• NCBI GeneID: 1025
• NCBI Accession #: NP_001252.1
• NCBI GenBank Nucleotide #: NM_001261.3
• Molecular Weight: 53,365 Da
• NCBI Official Full Name: cyclin-dependent kinase 9
• NCBI Official Synonym Full Names: cyclin-dependent kinase 9
• NCBI Official Symbol: CDK9
• NCBI Official Synonym Symbols: TAK; C-2k; CTK1; CDC2L4; PITALRE
• NCBI Protein Information: cyclin-dependent kinase 9
• UniProt Protein Name: Cyclin-dependent kinase 9
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: CDK9
• UniProt Synonym Gene Names: CDC2L4; TAK
• UniProt Entry Name: CDK9_HUMAN

NCBI Description

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. [provided by RefSeq, Jul 2008]

Uniprot Description

CDK9: a protein kinase of the CDK family. Forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. A component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. Transcriptional elongation factor and cofactor for HIV Tat protein; RNAi blocks HIV replication, and inhibitors also block varicella zoster replication. Mediates signals leading to cardiac hypertrophy. Inhibitor: Flavopiridol.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; Cell cycle regulation; EC 2.7.11.23; Kinase, protein; EC 2.7.11.22; Nuclear receptor co-regulator; CMGC group; CDK family; CDK/CDK9 subfamily; CDK9 subfamily

Chromosomal Location of Human Ortholog: 9q34.1

Cellular Component: nucleoplasm; transcription elongation factor complex; PML body; transcription elongation factor complex b; intracellular membrane-bound organelle; membrane; cytoplasm

Molecular Function: RNA polymerase subunit kinase activity; protein binding; snRNA binding; DNA binding; cyclin-dependent protein kinase activity; chromatin binding; ATP binding; protein kinase activity

Biological Process: transcription from RNA polymerase II promoter; regulation of histone modification; transcription initiation from RNA polymerase II promoter; positive regulation of histone phosphorylation; viral reproduction; transcription, DNA-dependent; positive regulation of viral transcription; regulation of mitotic cell cycle; DNA repair; protein amino acid phosphorylation; regulation of DNA repair; cell proliferation; transforming growth factor beta receptor signaling pathway; RNA elongation from RNA polymerase II promoter; replication fork arrest; positive regulation of transcription from RNA polymerase II promoter; gene expression