Rat Cyclin Dependent Kinase 5 ELISA Kit | CDK5 elisa kit

Rat Cyclin Dependent Kinase 5 ELISA Kit

Cat. Num. AAA726515

• Gene Names: CDK5; PSSALRE
• Reactivity: Rat
• Synonyms: Cyclin Dependent Kinase 5; Rat Cyclin Dependent Kinase 5 ELISA Kit; CDK5 elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 1.0-25ng/mL
• Sensitivity: 0.1ng/ml
• Intended Uses: This CDK5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CDK5. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CDK5 elisa kit

Principle of the assay: CDK5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CDK5 antibody and an CDK5-HRP conjugate. The assay sample and buffer are incubated together with CDK5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CDK5 concentration since CDK5 from samples and CDK5-HRP conjugate compete for the anti-CDK5 antibody binding site. Since the number of sites is limited, as more sites are occupied by CDK5 from the sample, fewer sites are left to bind CDK5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDK5 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CDK5 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 4826675
• NCBI GeneID: 1020
• NCBI Accession #: NP_004926.1
• NCBI GenBank Nucleotide #: NM_004935.3
• Molecular Weight: 29,544 Da
• NCBI Official Full Name: cyclin-dependent kinase 5 isoform 1
• NCBI Official Synonym Full Names: cyclin-dependent kinase 5
• NCBI Official Symbol: CDK5
• NCBI Official Synonym Symbols: PSSALRE
• NCBI Protein Information: cyclin-dependent kinase 5; TPKII catalytic subunit; protein kinase CDK5 splicing; cell division protein kinase 5; serine/threonine-protein kinase PSSALRE; tau protein kinase II catalytic subunit
• UniProt Protein Name: Cyclin-dependent kinase 5
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: CDK5
• UniProt Synonym Gene Names: CDKN5; TPKII catalytic subunit
• UniProt Entry Name: CDK5_HUMAN

NCBI Description

This gene encodes a proline-directed serine/threonine kinase that is a member of the cyclin-dependent kinase family of proteins. Unlike other members of the family, the protein encoded by this gene does not directly control cell cycle regulation. Instead the protein, which is predominantly expressed at high levels in mammalian postmitotic central nervous system neurons, functions in diverse processes such as synaptic plasticity and neuronal migration through phosphorylation of proteins required for cytoskeletal organization, endocytosis and exocytosis, and apoptosis. In humans, an allelic variant of the gene that results in undetectable levels of the protein has been associated with lethal autosomal recessive lissencephaly-7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015]

Uniprot Description

CDK5: a protein kinase of the CDK family. Unlike other members of this family, it is not activated by cyclins but by p35 (CDK5R1) and p39. An important regulator of neuronal positioning during brain development. May also play a role in synaptogenesis and neurotransmission. Substrates include TAU, MAP2, NF-H and -M, Nudel, PDE6, beta-catenin, amphyphysin, dynamin I, synapsin 1, Munc-18, and NMDA receptor 2A. Plays a role in myogenesis, haematopoietic cell differentiation, spermatogenesis, insulin secretion, and lens differentiation. Implicated in the pathology of neurofibrillary tangles and formation of senile plaques, hallmarks of Alzheimer?s disease. Induces tau phosphorylation and aggregation and neurofibrillary tangle deposition and neurodegeneration in in vitro and in vivo animal models. Brain samples from Alzeimer?s pateints show elevated CDK5 activity.

Protein type: Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Cell cycle regulation; CMGC group; CDK family; CDK/CDK5 subfamily; CDK5 subfamily

Chromosomal Location of Human Ortholog: 7q36

Cellular Component: cyclin-dependent protein kinase 5 activator complex; dendrite; postsynaptic density; perikaryon; cytosol; postsynaptic membrane; growth cone; cytoskeleton; membrane; cell soma; lamellipodium; axon; cytoplasm; cell junction; nucleus; neuromuscular junction; filopodium

Molecular Function: acetylcholine receptor activator activity; ErbB-2 class receptor binding; protein serine/threonine kinase activity; protein binding; ErbB-3 class receptor binding; ephrin receptor binding; p53 binding; cyclin-dependent protein kinase activity; tau-protein kinase activity; cytoskeletal protein binding; kinase activity; ATP binding; protein kinase activity

Biological Process: Schwann cell development; negative regulation of synaptic plasticity; axon extension; cerebellar cortex formation; rhythmic process; regulation of synaptic plasticity; motor axon guidance; sensory perception of pain; receptor clustering; regulation of cell migration; regulation of apoptosis; neuron differentiation; corpus callosum development; receptor catabolic process; oligodendrocyte differentiation; neurite development; central nervous system neuron development; synaptic transmission, glutamatergic; skeletal muscle development; dendrite morphogenesis; cell division; synaptic vesicle endocytosis; negative regulation of protein ubiquitination; negative regulation of transcription, DNA-dependent; synaptic transmission, dopaminergic; regulated secretory pathway; axon guidance; negative regulation of protein export from nucleus; positive regulation of protein binding; cell-matrix adhesion; protein amino acid autophosphorylation; neuron migration; nucleocytoplasmic transport; negative regulation of axon extension; negative regulation of cell cycle; intracellular protein transport; synaptic transmission; synaptogenesis; behavioral response to cocaine; positive regulation of neuron apoptosis; visual learning; cortical actin cytoskeleton organization and biogenesis; layer formation in the cerebral cortex; serine phosphorylation of STAT3 protein; negative regulation of proteolysis; hippocampus development; peptidyl-threonine phosphorylation; cell cycle; cell proliferation; positive regulation of calcium ion-dependent exocytosis; peptidyl-serine phosphorylation; embryonic development; synaptic vesicle exocytosis; neuron apoptosis; positive regulation of protein kinase activity; regulation of excitatory postsynaptic membrane potential; blood coagulation; phosphorylation

Disease: Lissencephaly 7 With Cerebellar Hypoplasia